FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325857
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC774541.224403270576619No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG247100.3906190101989343No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA205470.32480974514599364No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC202840.3206522057011406No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT195190.30855898260109266No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG136290.21544906879810913No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA135290.21386825532097864No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT126830.20049457330445503No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA117450.18566654288897141No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG117080.18508164190243315No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA111270.17589711560030524No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC106740.1687360305489043No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT104130.1646101073735938No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC104030.16445202602588077No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA94550.14946591426268407No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT84650.13381586083909264No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA84400.13342065746981002No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA77580.12263950955578036No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG77320.12222849805172643No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC76590.1210745042134212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT76000.12014182426191423No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG75210.11889298161498119No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT70640.11166866402449502No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC68720.10863350214840456No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA66270.10476050912943495No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT63400.10022357445007056No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)