FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9217486
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA7035957.633263560150782No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA6212296.73967934423768No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC1535631.6659965634881355No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1111691.2060663829595186No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA809020.8777013602190445No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT703020.762702541669171No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA550230.5969415087801598No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA488730.5302204961309407No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC452470.490882221030767No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA441040.4784818767286438No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAAT435740.47273193580115014No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA348040.37758668686884905No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT297180.32240895185520213No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA283790.307882214304421No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG278880.3025553822376297No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA264470.28692205228193457No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA210260.22810992064430582No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT207130.2247142008135407No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA198750.21562278478101296No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA186850.20271254005701772No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC169000.18334717297102485No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC157010.1703392877407137No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA153190.16619499069485974No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT120120.13031752909632843No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT118840.12892886411761298No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA115760.12558738901257893No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA105510.11446722023770906No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACAACTGTAGGCACCA100590.10912953922577154No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC96090.10424751390997501No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT94170.10216451644190185No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATCA92640.10050462783453103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)