FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9442325
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA3274903.468319508171981No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC2104342.228624835514558No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA902920.9562475343731549No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA873940.9255559409361571No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA678930.7190284172595203No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC671020.7106512432054606No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC581990.6163630249964919No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA424840.44993155817026No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA280470.29703489341872896No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT244780.25923699936191563No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC241650.25592213782092865No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT227610.24105291864027134No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG220950.23399957108021593No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC217620.23047289730018825No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC195090.20661224857225313No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACAACTGTAGGCACCA194100.20556377798900166No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG185330.19627581130706684No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA168650.17861067057107227No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC165320.17508399679104458No Hit
TTTGTCAAAGTTAAATTATAGGCTAAATCCTATATATCTTACCAAACTGT146740.15540663978416333No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT145120.15369096064793364No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT142010.1503972803308507No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG137200.14530319598192182No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA136040.14407468499548576No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATCA127710.1352527052394405No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAAACTGTAGGCACCAT126090.1335370261032108No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA121300.128464122978186No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA118240.12522339572086325No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT116150.1230099578228879No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA111030.11758756450344593No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA103470.10958106186770737No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAACTGTAGGCACCATC100800.10675336847651398No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA98520.10433870895144998No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA98020.10380917835384823No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT95060.10067435721604584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)