FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6649645
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC872071.311453468568623No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG704231.059049016902406No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC636980.9579157985125523No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA416060.6256875367030871No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT364490.5481345244746149No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA337150.5070195476600631No Hit
AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTA275500.4143078314707026No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC268070.4031343026582622No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA260910.39236681055906No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG237630.3573574228398659No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG214310.3222878815335255No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC209490.3150393742823865No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA196470.2954593816662393No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT193330.2907373250752484No Hit
ATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAAC178050.2677586547853307No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA162160.24386264229143062No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC156040.2346591434580342No Hit
GTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAGA153330.23058373792886688No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT150640.2265384091932727No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT147710.22213215893480026No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA139930.2104322862348291No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG137000.2060260359763566No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA134780.20268751188973247No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA132790.1996948709292No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC132540.1993189110095351No Hit
CACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGGA127890.19232605650376824No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA123410.18558885474337353No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT119850.18023518548734557No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA119010.17897196015727157No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG114190.17172345290613258No Hit
CACAATGATGACTTAAATTACTTTTTGCCGTTTACCCAGCTGAGGTTGTC112670.16943761659457007No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC105930.1593017371604048No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC103000.15489548690193236No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT94790.14254896314013754No Hit
TGTTATGATGATGGGCGAAATGTTCAACTGCTCTGAAGGGGCTGAATGAA94490.1420978112365397No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT93790.14104512346147802No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC87410.13145062631163018No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC85980.12930013557114703No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT85810.1290444828257749No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG84460.12701429925958452No Hit
ACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACATT83960.12626237942025478No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA83280.12523976843876627No Hit
ATCAATGATGAAACCTATCCCGAAGCTGATAACCTGAAGAAAAATAAGTA80270.12071321100600106No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA79050.11887852659803644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA76580.11516404259174738No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC76560.1151339657981742No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG76010.11430685397491144No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA75730.11388577886488677No Hit
TAGAATGATGAGGATCTTAACCACCATTATCTTAACTGAGGAACTGTAGG74980.11275789910589211No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA73300.1102314484457441No Hit
TGGCGATGAGGAGGTACCTATTGTGTTGAGTAACGGTGATAATTTTATAC73190.11006602608109155No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG69240.10412585935038636No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA68500.1030130179881783No Hit
CTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGA67830.10200544540347643No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC67450.1014339863255858No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA67430.10140390953201261No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA67080.10087756564448178No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT66950.10068206648625602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)