FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9732534
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA4962685.099062587400157No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATCA3018633.101586904294401No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA2429072.4958248283540545No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1642941.6880906863515708No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1526041.5679780825836314No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA953670.979878416042523No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTAACTGTAGGCACCATCAA950750.9768781696524256No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA767930.7890339761463971No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA673000.691495144019019No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA491490.5049969514619729No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA488890.5023254991968177No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT452740.46518203789475593No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT396650.40755059268223465No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC377070.3874325021623351No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC361680.3716195597158972No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAAT355890.36567044101772467No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA350840.3604816587334809No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA320780.329595560621725No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA307710.31616637558111793No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC262800.27002217510876403No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA256510.2635593155903694No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA244800.25152750558076653No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA241000.24762307534707817No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA213220.21907963537553526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA199120.20459214424527056No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT182790.187813369056815No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT171580.17629529986743434No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT161260.16569168933804906No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAACTGTAGG159410.1637908483032271No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT153210.1574204621324724No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG144850.14883071561835798No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATCAA129260.1328122768438312No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA126090.12955516004362277No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT122550.1259178750364499No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATC111030.11408128653853147No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG108550.11153313207022961No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAACTGTAGGCACCAT100590.10335437821229292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)