FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7591872
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1311761.7278478878463703No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT807241.0632950608229432No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA659390.8685473095436803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC657330.8658338812877772No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA623040.820667155610632No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA587890.7743676394965563No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT471600.6211906628562758No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC446100.5876021092031056No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC356840.4700289994351854No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG353800.4660247169604546No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA300120.3953175185250752No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA286140.37690308793404315No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC261230.3440916812085346No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA239100.3149420854303128No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT197950.2607393802213736No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG193220.25450903281825615No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA166310.2190632297277931No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG163730.21566485841700175No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC161170.21229283106985997No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA160680.21164740396044612No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA160240.2110678367601561No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA151930.20012191986377012No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA150490.19822515448100284No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT146860.19344372507861038No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA146030.19235045058715428No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC144070.18976874214949882No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA139440.18367011456462912No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT134850.17762417490705848No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG126340.16641481837417701No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT125580.1654137477554943No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAACTGTAGGCACCA124110.16347746642725272No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA122220.1609879618623707No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC121610.16018447097105956No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC119890.1579188900971987No Hit
ATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAAC117660.1549815381502744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT116710.153730199876921No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG116680.1536906839314467No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA113440.14942296182022036No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA105080.13841118501471047No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC100890.13289212463013075No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG99810.13146955059305532No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT99790.13144320662940576No Hit
AGCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTC96890.1276233319002217No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC95640.12597683417212513No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA92990.12248625898856041No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA92920.12239405511578699No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT92820.12226233529753926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG92700.12210427151564199No Hit
AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTA91760.12086610522411337No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG90580.1193118113687902No Hit
CACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACAT89440.11781020544076612No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA88600.11670375896748522No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT88280.1162822555490925No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT84060.11072367921903846No Hit
ACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACATT82810.1090771814909419No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATCA81660.10756240358109305No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA81210.10696966439897827No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC80860.10650864503511123No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG80830.10646912908963693No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAACTGTAGGCACCAT80120.10553391838007806No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA78280.10311027372431991No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA78040.10279414616052537No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT76440.10068662906856174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)