FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7820110
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA3500494.476266957881666No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA2793263.571893489989271No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC753490.9635286460164881No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC678830.8680568431901853No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA576400.7370740309279536No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA574420.7345420972339264No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA393940.5037525047601632No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC342510.43798616643499894No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA324010.41432921020292557No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA314960.4027564829650733No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA312260.3993038461095816No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA274840.35145285680124705No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA244930.31320531296874343No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA215340.2753669705413351No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT204320.26127509715336483No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT198390.2536920836151921No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAAT196110.2507765236038879No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC195090.24947219412514657No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTCAACTGTAGGCACCATC145390.18591810089627897No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC140160.17923021543175224No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA130680.16710762380580324No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTTCAACTGTAGGCACC113230.14479335968419882No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAACTGTAGGCACCATC104760.13396230999308192No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC100260.12820791523392894No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA97690.12492151644925711No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC88400.11304188815758347No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAACTGTAGGCACCATC88360.11299073798194655No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC78920.10091929653163445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)