FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896854
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC381170.778397722292721No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT370610.7568328563604305No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC270340.5520687363764573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA246500.5033844178323471No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC223860.4571506522350881No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG214130.4372807520910364No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA208100.42496672353310916No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA205390.419432558128137No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT164900.3367468174464666No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG148840.303950250507775No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC143300.29263686440314535No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT121980.24909870704742268No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG117050.23903101869077575No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT111640.22798310915538833No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA110030.22469528395169633No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA106800.21809921226975523No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA105980.2164246677560736No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT100690.20562181351537132No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC97150.19839268232216034No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT96890.1978617291836759No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA94780.19355284025212924No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA94200.19236840632781782No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA89390.182545773265856No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG86830.17731792697924015No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT86330.1762968632513855No Hit
ATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAAC84130.17180418284882498No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT80130.16363567302598772No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA80050.16347230282953096No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC77590.15844866928848603No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT77050.157345920462403No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG76670.15656991202923348No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG72880.14883024897209515No Hit
TGTTATGATGATGGGCGAAATGTTCAACTGCTCTGAAGGGGCTGAATGAA70650.14427630474586337No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC70290.14354113886180803No Hit
AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTA68730.14035542003090148No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA66990.13680211825796726No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA63370.12940961686829952No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA62290.12720411921613345No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT61610.12581547254625114No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT59640.12179248145850377No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA57610.11764696272341384No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG56030.11442040134339312No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA55490.1133176525173101No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC55260.11284796320249695No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA52820.10786517221056621No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA51520.1052104065181441No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC50350.1028211173949642No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA49930.10196342386356629No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA49160.10039098572267009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)