FastQCFastQC Report
Mon 14 Nov 2022
EGAF00007294396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996612
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA110740.5546395594136467Illumina PCR Primer Index 9 (96% over 28bp)
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG58350.292245063136954No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG57820.28959056641951464No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA54380.2723613801780216No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA52570.2632960234637476No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT49530.24807023097126532No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG46540.2330948626974094No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT45340.22708468145037694No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA44680.22377908176450909No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA44380.22227653645275094No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC43400.21736822176767445No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG40910.20489709568008208No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA40130.20099047786951096No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC39450.19758470849619258No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG39200.1963325874030608No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCAGCAGCC37760.18912036990662182No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC37030.18546417631467707No Hit
TGCAGATATACACTTCAGATAACTACACCGAGGAAATGGGCTCAGGGGAC36540.1830100189721388No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA35800.17930374053646878No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC35620.17840221334941392No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT34840.1744955955388428No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA33980.17018829897846952No Hit
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATCACC33880.1696874505412168No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC33110.165830917574371No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC32870.16462888132496448No Hit
CAGGAAGATTTTATTGAAATTAGCATTTTCTTCACGGAAACAGGGTTCCT32750.16402786320026125No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA32740.16397777835653599No Hit
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC32640.16347692991928328Illumina PCR Primer Index 9 (96% over 28bp)
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC32190.1612231119516461No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC31780.15916963335891No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA31610.1583181910155804No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT30760.1540609792989324No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA30530.15290902789325117No Hit
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT30280.1516569068001194Illumina PCR Primer Index 9 (96% over 28bp)
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG29590.14820105258307573No Hit
GCTGCCTTCTTTGCCTTTGTAACCTTCGGCTTCCCCGACTTAGGCTTGGC29240.14644808305269127No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT28410.1422910410234938No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT26950.1349786538396043No Hit
CCAACAAGCAATAAAAACTGTACAATATTGGTCAGTCTTTTATATCTGAA26930.13487848415215375No Hit
GCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTCCTC26720.13382670243392306No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA26590.13317559946549454No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC25170.12606355165650612No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG24940.1249116002508249No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24720.12380973368886895No Hit
CGTTTCTCTGGCCGCGCTTAAGAAAGCGCTTGCGGCTGCTGGCTACGATG24700.12370956400141842No Hit
CATTTTCCACTTCTTCCTCAGTATTTAAGTTCTGACAAACTGCATGCATC24560.1230083761892646No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC24550.12295829134553934No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC24370.12205676415848449No Hit
GCCGGCTTCTTCGCTTTCTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGT23540.11789972212928701No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA23410.1172486191608585No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG23370.1170482797859574No Hit
ATTGGGATCAGCATCGACTCCTTCATCCTCCTGGAAATCATCAAGCAAGG23330.11684794041105633No Hit
AACCATGATGTGCTGAAACTGGAACACAACCACCCACAAGTCATTGGGGT23030.11534539509929821No Hit
GTTCTCAAACTCACACCCTTGCTTGATGATTTCCAGGAGGATGAAGGAGT22820.11429361338106753No Hit
CGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTAAACC22620.11329191650656212No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC22230.11133860760127656No Hit
GCAGGCGCCGCTAAAGCTAAGAAGCCCGCGGGGGCCACGCCTAAGAAGGC22140.11088784400774913No Hit
GAAAAAGGCAGTGAAGAAGACTCCGAAGAAGGCGAAGAAGCCCGCGGCGG21820.10928512900854047No Hit
ATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGT20540.10287426901170584No Hit
GGGCTTTGTTGCGGTTTTCACACGCCAGCTTCCTACTTCTTTTTGGCAGC20420.10227325088700259No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC20080.10057036620034338No Hit
TACCTGATGAATAATTTCTGCCACCAAAGTCATGTTGTTTTTTCTAACCA20020.10026985713799176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)