FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292563
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA157110.6853028684489805Illumina PCR Primer Index 9 (96% over 28bp)
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG59730.26053809644489595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48260.21050675597573545No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG48170.21011418224929918No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC46420.20248080423525983No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG44900.19585067018877997No Hit
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC43020.1876502412365549Illumina PCR Primer Index 9 (96% over 28bp)
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA42480.18529479887793704No Hit
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT40020.17456445035534465Illumina PCR Primer Index 9 (96% over 28bp)
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA36650.15986474526545183No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA34510.15053021443685516No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG33240.1449905629638095No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG32450.14154463803175746No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC29610.1291567559975451No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC28450.12409691685681047No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC27320.11916793562488795No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA26210.11432619299884016No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC25520.11131646109616182No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC25520.11131646109616182No Hit
CTAGAAGGTGATCTACTTCTATACATTGAGGCTGTCTTTTTTTTTTTTTT23280.1015457372381915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)