FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334647
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA575022.4629847681469617Illumina PCR Primer Index 9 (96% over 28bp)
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC137500.5889541331087741Illumina PCR Primer Index 9 (96% over 28bp)
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT120040.5141676664609254Illumina PCR Primer Index 9 (96% over 28bp)
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG52810.22620122014163171No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC43000.18418201980856208No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA40820.1748444197345466No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG38580.16524982149335637No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC37340.1599385260384118No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA33020.14143465800183067No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG32410.13882184330222086No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA31900.13663735888123557No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA31390.1344528744602503No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT30630.13119756434270363No Hit
GGTTGTTTTCATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCA29660.12704276064004538No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29430.12605760099920887No Hit
CTAGAAGGTGATCTACTTCTATACATTGAGGCTGTCTTTTTTTTTTTTTT28700.12293078996524957No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC27300.11693416606450568No Hit
TGTGGCCAGTACTGAGTTTTGTTATCCTTCCTTTAGGTAAGGAGTGCCAA25190.1078963971855274No Hit
CCCGCCACCTGAAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA24880.10656857332179126RNA PCR Primer, Index 26 (96% over 28bp)
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC24720.10588324487599196No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC24040.10297059898134495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)