FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293807
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT92920.17552585502267085No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG90890.17169118556834428No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT87370.1650419065145367No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG78070.14747420901442004No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG72840.13759474042026842No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA72600.13714138048478156No Hit
TTTTCCCTGTCCATTCCATCAAGTCCTGAAAAATCAAAATGGATTTAGAG72560.13706582049553376No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC70290.13277779110572033No Hit
GGTTGTTTTCATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCA69330.1309643513637728No Hit
CCGCTTCACATAGGTCTTTTCCCGAGACAGGCATTTGTCGATCTCCTTGG69010.13035987144979028No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT65120.12301166249544043No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG64040.12097154278574945No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG61190.11558789355184274No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC59680.11273550395773779No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC59440.1122821440222509No Hit
GCCGGTGCAGTTACACGTTTTCCTCCAAGGAGCCTCGGACGTTGTCACGG58910.11128097416471738No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA58580.11065760425342291No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC57030.10772965467007013No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC56080.10593510492543456No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG55890.10557619497650747No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG53030.10017365574528879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)