FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7633653
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACC179490.23512989128533873No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG161950.21215268757959002No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA137690.1803723590789364No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC132570.1736652163780565No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC131730.17256482577869336No Hit
TTTTCCCTGTCCATTCCATCAAGTCCTGAAAAATCAAAATGGATTTAGAG129180.16922435431634106No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG123190.16137752135183508No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC122430.16038192985717323No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG121270.15886234283900513No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG117560.15400228435848473No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC116130.1521290003619499No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT112750.14770123818832218No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA112220.14700694411967638No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA105430.13811212010815793No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC105040.13760122447273934No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC104490.13688073062791825No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA103600.13571484058811686No Hit
GGTTGTTTTCATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCA99730.13064518389819396No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC98000.12837890325902945No Hit
CCATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCT93890.12299484925500281No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA92370.12100366626567909No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC83070.10882077034415896No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG82020.10744528209495506No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT81960.10736668276642913No Hit
CCAACAAGCAATAAAAACTGTACAATATTGGTCAGTCTTTTATATCTGAA81770.10711778489276368No Hit
AACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGCCCA81700.10702608567615007No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA79770.10449780727523246No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT79560.10422270962539167No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA78880.10333191723543105No Hit
GACAATATCCGTAATATCACAGCGTGTATTTCTGTCTTTCCAAGGATGTG77720.10181233021726295No Hit
GCAGCAGGAAGATAGGCACGATGATGAAGAGGATGATCAGCAGCGTCTGG77600.10165513156021107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)