FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021015
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG86780.4293882034522257No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA82740.4093982479100848No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC58060.2872813907863128No Hit
AAACAAACTGTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA55390.27407020729682857Illumina PCR Primer Index 9 (96% over 28bp)
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC55330.27377332676897503No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC54830.27129932237019516No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA53320.2638278290858801No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG51410.2543771322825412No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA48220.23859298421832595No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA44720.22127495342686718No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG43470.21508994242991764No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC42860.21207165706340625No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC40990.20281888061196973No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC37750.18678733210787649No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA37190.1840164471812431No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA35870.17748507556846438No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA27330.135229080437305No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG27020.13369519771006153No Hit
CTAGAAGGTGATCTACTTCTATACATTGAGGCTGTCTTTTTTTTTTTTTT25940.1283513482086971No Hit
GCGGGAAGCTGTGGATGATGAAACTAAGGGAGTGGAAGGAACAGATGGTG24690.12216633721174758No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT23110.11434848331160334No Hit
CCTGGTCTGTATGGTTTCCTTTTTCTCTTTTTGACACCATCTGTTCCTTC22610.11187447891282351No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA21580.10677802985133707No Hit
CATTTTCCACTTCTTCCTCAGTATTTAAGTTCTGACAAACTGCATGCATC21470.10623374888360552No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT20700.10242378210948459No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA20610.10197846131770423No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT20590.10187950114175304No Hit
GATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCCTCCAGCTTCGGTCGA20420.10103833964616789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)