FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10703999
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA1166121.089424615977636No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA1075321.0045965064084927No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG849750.7938621817883204No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG773700.722813968872755No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC717620.6704223346807114No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC700850.6547552928583046No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT669510.6254765158330078No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC662050.6185071579322831No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA662040.618497815629467No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC631150.5896394422308896No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG625150.5840340605412987No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT618900.578195121281308No Hit
TGGGACTCTTAAAGATTTATACCACCCAGATTTTCATTCTTCTTTCTGTT603960.5642377208742265No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT593570.5545310682484181No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC583680.5452915307634091No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG565950.5287276278706678No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC547150.5111640985766162No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA543510.5077635003515976No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG514630.48078292981903303No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT511880.4782137965446372No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA505540.47229077655930274No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG492500.46010841368725836No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC466530.43584645327414545No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC466330.4356596072178258No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA462970.43252059347165484No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC458910.428727618528365No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT438840.4099776167766832No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC433230.4047365848969156No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA403350.37682178408275263No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA386540.3611173730490819No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC383330.35811849384515076No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC380480.355455937542595No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA378710.3538023499441657No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT376310.3515601972683293No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT375030.35036438250788327No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA374110.3495048906488126No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA363270.33937783439628494No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA358550.3349682674671401No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC356660.33320257223491895No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA350940.32785877502417554No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA335430.3133688633565829No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT334960.31292977512423165No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA333900.3119394910257372No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT324080.30276534966044No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA323630.3023449460337207No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG321890.30071938534373926No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA309510.28915361445754995No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA287240.26834830608635146No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG276470.2582866459535357No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT262180.2449364952294932No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT250120.23366967803341537No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT247790.23149292147729086No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA246550.23033447592810874No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT246010.22982999157604556No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC229780.21466743410570197No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC227090.21215435464820204No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC225260.21044471323287678No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT201600.18834082477025643No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT198780.18570629537614866No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT197490.18450113831288661No Hit
TCAGTGGACAGGAAACGCACCATATCCCCCTGCCTGGATGGGTGTTTTTG196690.18375375408760783No Hit
TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAA177270.16561100201896506No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA173950.16250935748405806No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT167860.15681989506912325No Hit
GATGTGCAGAGAAGCCAGCAAGTAGTTGAGTGTGACGGGTGCATCGGTTC166380.15543723425235748No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC160280.14973842953460664No Hit
GCGTCTGGAGTTTTTGGGTGTTAATGATTCTGCCTGCCCACAGGTCGGGT154010.1438808056689841No Hit
GCCGTGATGAATATCGAACAGAGTTTACCACAGCTTTGCAGCGCGTTGAC152600.14256354097193022No Hit
GGTCACTGATGGAGGAGGTCTTGCCAGCAAAGCAGTAGTTGGGGTTGTAG148270.13851832385260873No Hit
CCTCACAGGCTCATACATAGAAAGAGATGTGACTCCCGCCATCATGGAGG146800.13714500533865895No Hit
GCTACTGCTGGTCTTACCTGAACACATCTGCAGATTCGTTTTCCCTGTCT146260.13664052098659576No Hit
CTCTTTTTCAGCCACAGGAAAAACCCAAGTAGTAATAACAGTGCTGTTGA144380.13488416805719058No Hit
CCACTGTCTTTTCCTTTCCTGACCTTTATGGTTGTAATTGCTAAGAAAAA139610.1304278896139658No Hit
CAGCTGTTACAAACACGAGCCACAACTTTAAAAGAATAAAAAATTAAAAG139060.1299140629590866No Hit
GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGA136880.12787744094520187No Hit
AGCAACAATTTTATTTACTTCTTGACGGTCCAAAGGGAAACTCTAGATGC136360.12739164119877067No Hit
GTGAATTAGTTCGCTACGATGCAAGAGTACACACTCCTCATTTGGATAGG133460.12468237338213503No Hit
GGAAAGGCGGGAAGAACACCATGATGCGGTCCCACGGCGTGTCTGTGCTG131900.12322497414284138No Hit
ATTAAACATATACTTACCATATCGGCTGCGATGAGACAGGAAAGGGAAGG130130.12157138654441205No Hit
GACTACATGGACATGAAGCAGGCTGATACTACACAGTATGTCCCCATGCT128730.12026346415017417No Hit
CTTACCTTCTTCCCAGTGATAACCAGTGTGTAGCCATTTTGGTTTAATGT127530.11914238781225595No Hit
GGTACCAGGAGATGATGTAAGGGACAAGCAGCCACACCCCATTCTTGAGG127110.1187500110939846No Hit
GTCAACCATGCAGAGTGAAAGGATATCCCAATAGAATTACCCGCCAAGCA127020.11866593036864073No Hit
GCCAATTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAA126230.11792788844617792No Hit
GGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGC125850.1175728809391705No Hit
TGGTTGGCCGTTATATTGTTCTCCTGTGTCTGTTCTAGCACTCGGGGATA125050.1168254967138917No Hit
GAAAGAGTCTGGATTTTTAGGGCTCATACTATCCTCCGTGGTCATGCTCC124720.11651720072096419No Hit
CCTAAAATTCAATTTGTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGA122560.11449926331271144No Hit
GCGTCAGTCTGGTGTGCTAACTCTATGGCCTGCTTATCTGTTCCTCCTCC121640.11363977145364082No Hit
GGAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTT120930.11297646795370589No Hit
GCATGTTTCCAATTTTAGCGAGTGCCCATTTGACAGAACGGGAAGCCCTC120630.11269619886922635No Hit
GGAATTGGCCCTATACTTAGGCCCTTTTTCTCTCTAGATGTAATAATGAA120520.11259343353825053No Hit
GTTGTAAGCCTTACATTCAACCGTGCCATTGTGCTTGAATGCACTAGAAT119750.11187407622141968No Hit
CAGTGAAGGTTCTGTTCATATAGATCCACTGCTGGTGTTCAGGTTTGGGG119090.11125748423556467No Hit
CGCATGTGAGGATCCTGGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCC117900.11014575020046247No Hit
GTAGAGATCCCATCCTGCCAAAGTTTGTGATTCCACATTTCTCTTCCATT117780.11003364256667064No Hit
ATTTTGAGAAATTCCCTTAATATCCCCATGCTCAAAAAATCCCCGTGCTC116660.10898730465128033No Hit
TTGTTGCTACTCTCCTGAACTCTCTCACTCATTTGTTTCAGTGGACAGGA116380.10872572017243275No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT115580.10797833594715395No Hit
CACTGAGCGTTTGTTAGTCCTGGTGTTTTATTGTTTGGCTTTTAGGTGGC113590.10611921768677296No Hit
GCTTGAGAAAGTTGGAAACGTTGCCTTAGAAGCCTGTTTTTTCTCCTTTT113420.1059603985389012No Hit
CCTGATAATGGCGTGCTTGTGATTCTTTTCCTCTCTCCTAGACCATGCTC113380.10592302932763728No Hit
CACCTCGACCATCATGACCGACTACAACCCCAACTACTGCTTTGCTGGCA113360.1059043447220053No Hit
CATTGAGGAGGGATAGTAAATGGCCCTTGTCTTGCAGGTATGACATAATG112960.10553065260936591No Hit
CTAGGATTGTTAAATAACCGCCTTTGCTTTGATCTTTTCAGGTACGAAAC112870.10544657188402203No Hit
CGGGACAAGGATGCCTGACCAGTTAGAGGGCCCACAGAGGAGGACTCACG112330.10494208753195884No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC112130.10475524147563915No Hit
CCCTGGTCATTACAGAATATTGTTGCTATGGTGATCTTTTGAATTTTTTG111680.10433483784891982No Hit
GCCAAGAAACCATATGCTCACCTTGTTACATCACCACACATGGCAATCCC111550.10421338791231202No Hit
TTTGGCCACAAACCTAACGTGCTTTTTGTTTCTACAGACAAACCAGTTAA110240.102989546243418No Hit
CATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGGGGAGTGTA109600.10239163886319497No Hit
ACAGACATGTCCCCCATCCTAACTAGTGGGGACTCTGATATATCCAGTCC109160.10198057753929163No Hit
CTTTTTAAAGACTCAGAGCAGGCCTATTTTGAAGGGATGGCTGGCTTACA107720.1006352859337898No Hit
CATGTCGCCCAAGAGCAGAAGTCAAACCATACCCAATAGAGTCCGAGGCG107360.10029896303241433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)