FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18587599
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA3054911.6435204998773645No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA3016241.6227163067161068No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG2170681.1678108614243292No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG2071181.114280548014835No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC1955761.052185384459822No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC1949971.049070404413179No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT1845210.992710247299826No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA1792810.964519408881158No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC1732120.9318686076668643No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT1692520.9105640809229854No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC1680350.9040167049009395No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG1604130.8630108708499683No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC1584500.8524500663049596No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT1561760.8402161032202168No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG1490060.8016419979794055No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC1477930.7951161416813436No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA1451240.7807571058532089No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT1422310.7651929654819861No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG1408860.7579569582924616No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA1394640.7503066964162505No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG1355000.7289806499483876No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC1269650.6830629388981331No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC1253460.6743528306157239No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA1249270.6720986395284296No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC1242980.6687146629319903No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC1205830.6487282192821139No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT1201090.6461781319900435No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA1094540.5888549672284193No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA1067370.5742376947124801No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC1042190.5606910284647307No Hit
TGGGACTCTTAAAGATTTATACCACCCAGATTTTCATTCTTCTTTCTGTT1032740.5556069936735778No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA1025730.5518356620454314No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC1019250.5483494667600695No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA1014350.5457133005720642No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA1008970.5428188976962544No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT993400.5344423451355929No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA982810.5287449982109039No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT980290.5273892555999299No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC946920.5094364258665145No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA933290.5021035799190632No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA917590.4936570882554546No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT905710.48726573023229086No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA891060.47938413132325486No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA888140.47781319147244355No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT877660.4721750237887099No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG850330.4574716723768357No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA846630.45548109790834196No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA785190.4224268018693539No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG733340.3945318596554617No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT718070.386316705024678No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA688590.37045666844867914No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT677080.3642643678723648No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT653180.3514063327920943No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC643130.34599950214118563No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC620070.3335933812645732No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT605860.32594849931935804No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC591340.31813683951326904No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT561070.3018517883885918No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT557740.3000602713669474No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT555990.2991187834426598No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA481690.2591458961429069No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT455710.24516883541548318No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC433780.23337064674141075No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT324480.17456800095590613No Hit
GGAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTT309920.16673482142583343No Hit
TCAGTGGACAGGAAACGCACCATATCCCCCTGCCTGGATGGGTGTTTTTG284190.15289225897330794No Hit
CCTGAAAACAACGTTCTGGTAAGGACAAGGGTTGGGCTGGGGACCTGGAG263720.14187954022464117No Hit
TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAA231470.12452926276276995No Hit
GATGTGCAGAGAAGCCAGCAAGTAGTTGAGTGTGACGGGTGCATCGGTTC222710.11981644321033609No Hit
CCTCACAGGCTCATACATAGAAAGAGATGTGACTCCCGCCATCATGGAGG222390.11964428541846636No Hit
GCTACTGCTGGTCTTACCTGAACACATCTGCAGATTCGTTTTCCCTGTCT221830.11934300928269433No Hit
GCCGTGATGAATATCGAACAGAGTTTACCACAGCTTTGCAGCGCGTTGAC221240.11902559335393453No Hit
GGTCACTGATGGAGGAGGTCTTGCCAGCAAAGCAGTAGTTGGGGTTGTAG213530.11487766655607322No Hit
GCGTCTGGAGTTTTTGGGTGTTAATGATTCTGCCTGCCCACAGGTCGGGT197900.1064688344094361No Hit
CCACTGTCTTTTCCTTTCCTGACCTTTATGGTTGTAATTGCTAAGAAAAA187560.10090598575964545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)