FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458058
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG120850.22141574897152064No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA115800.21216337385934705No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA107260.1965167830755921No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG97990.17953272024591896No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC81150.14867925551542324No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC78160.14320111658762147No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA75210.13779626379932203No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG72990.13372888305694075No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC72120.13213490952276433No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC70770.1296615023145595No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG70600.12935003622167443No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG70190.1285988532917752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69390.12713313050172792No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC68480.1254658708280491No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG66680.12216799455044268No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG65410.11984115962124257No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC65020.11912661976109451No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT64290.11778914771517636No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC64150.11753264622691809No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT63100.11560888506498099No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA63050.11551727739060302No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG62380.11428973455393841No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG61990.11357519469379035No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA61300.11231100878737454No Hit
CTAGAAGGTGATCTACTTCTATACATTGAGGCTGTCTTTTTTTTTTTTTT60470.11079032139270047No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA60290.11046053376493983No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA57370.10511064558126719No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT56890.10423121190723882No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG56570.10364492279121987No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC55860.10234409381505291No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA55580.10183109083853634No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC55200.10113487251326389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)