FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526599
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA129460.8480288536806326No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA126160.8264121750374526No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG90600.5934760863854882No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC86190.564588343107784No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG84040.5505047494463183No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC83150.5446747967213393No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG81910.5365521659584476No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA80400.5266608978520227No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC77500.5076644226807433No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC72720.47635299119153096No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG69970.45833909232221426No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT69850.45755303128064406No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT69290.4538847464199833No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC60970.3993845142044506No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT59050.3868075375393276No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG58790.3851044052825922No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA58490.3831392526786668No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC58240.38150162550872885No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA58020.3800605135991835No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT57550.37698177451970033No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG54680.35818181460881343No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT52200.34193655308302967No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA51890.3399058953923067No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA49370.32339861351933286No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC48760.31940280322468445No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA47590.31173870806937515No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA46690.30584325025759873No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC46580.30512269430282607No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA45840.30027531787980993No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC45190.2960174872379715No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA43090.28226141901049323No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA43030.2818683884897082No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC41870.27426979842119636No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT41280.270404998300143No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA40370.2644440354015691No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC38910.2548802927291319No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA38480.2520635739968387No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC38270.2506879671740909No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT37600.2462991263586574No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC36590.23968311259210834No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG36340.23804548542217044No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA36310.23784897016177792No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT35820.23463922090869965No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT35800.23450821073510464No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA33940.2223242645907668No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA32880.21538072539023018No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG32090.2102058235332265No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT31330.20522743693661533No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG29760.19494313830940543No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA29760.19494313830940543No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG29360.19232293483750482No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA28510.18675500245971602No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA28450.18636197193893092No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT25720.16847908324320926No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC24540.16074948300110245No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC24200.15852231004998693No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT23960.15695018796684657No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT22540.1476484656415994No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC22350.14640386899244662No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT22190.14535578760368636No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT21320.13965684505230253No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA21140.13847775348994726No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT20360.13336835671974107No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC20340.13323734654614602No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT20220.13245128550457586No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC19940.13061714307424543No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA19320.1265558276927995No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA19160.12550774630403924No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC16960.11109662720858587No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC16840.1103105661670157No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16770.10985203055943309No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT16520.10821440338949521No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG15930.10434960326844182No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC15720.102973996445694No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC15500.10153288453614866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)