FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3563406
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAATCTGCGAAATCTGTACCTTATTCGCTTCCTTGTCAAAATATGA218070.6119706819823506No Hit
TCAGTGTCTCACTTTCTTTATGTTTCCCTCTTGACCACTGAATTCCACCG209950.5891834946677421No Hit
GGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGCCTG185120.5195029699113713No Hit
TTTTCGGCGAAAAGTCAGGATGTTAGCAGAGCCAGTCAAGACTTGCCGAC181710.5099334737607784No Hit
GTCTGAGCGAACAGTTTCCATCTGGTAACAATTCAAAAGATCTTTAAGAC177960.49940983429898245No Hit
ACATATACAAAAGGATAAACGTTAATCTGCCCTGTGCCTTTGCGTTAATT176190.4944426764730149No Hit
CCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGATG172180.48318939800853455No Hit
CCCAATCCAGTTAGTCCTTATCCAAACTCTTCACACACTTCAGATATCTA162380.4556876202150414No Hit
ACTAATAAGTGTGACATACTAACCTCGACAGCAAAAGTCAAAAAATATTT160790.45122559708324006No Hit
TCATTCTTTGGCCAGACTAAAGTGGAAGAATGTTTTCATGGTGAAAATCA159360.4472125825684752No Hit
CACTTCCTCCTGAATGTACCCCCAACATAGATGGACCAAATGCTAAATCT159270.4469600152213921No Hit
GGACATAAGTTTTTCAGTTTGGGAATCTGCTCTTTGTTGAAAATGAAATC157360.4415999748555175No Hit
GGCTCTGTAAATTCTACCCGTTTTTAATTTTACATGCTTTTAATTATAGG157270.4413474075084344No Hit
TTCCATCAAAATGAGTTTTAGAACTTTGCCCTGATGTTGACATTTTTCAT155750.43708182564658643No Hit
TGCAGTCACTGTGTGGCACTAGATTTCATTTTCAACAAAGAGCAGATTCC155420.43615574537394836No Hit
TAAGTTGTTACAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTA153530.4308518310852033No Hit
GAGTACAACCTCAGTGGGACAAAGAAGAACTTCAGCAGTCTTAAAGATCT150650.42276967597854415No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA149420.4193179222350751No Hit
GCCCATGCCAACTGTTTAGCAACTTCAAGTTTCCATAATATATTTATACA149100.41841990500100185No Hit
CTGTATCCTTCGCTGTTTCCATTCTTGGTTTAAGATTTCCGTTCTTTCCA147850.41491202518040327No Hit
GGGTAAGCCAAGAAAGAAATCCAGGTGAAAGCAGTCAACCAAATGTCTCC145950.4095800478530934No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATGAACTTTTCACATACATTG143220.401918838324906No Hit
TCCACAGGGATCTGGCAACGAGAAATATATTGGTGGAGAACGAGAACAGA142670.4003753712038426No Hit
GGATACACCTGTCAAGACTCAATATGATTTCCCATCTTGCAGATGTGTAG141150.3961097893419947No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC140520.394341817912413No Hit
GGCTTCTAGTTTCCTTTGTCGGCAAGTCTTGACTGGCTCTGCTAACATCC138460.3885608319680665No Hit
CATGTGATGCAAGTTTTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTG137680.386371914960013No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC137270.38522133037885664No Hit
GGAAATTGCCTCCTTATCCCACTCATGAGATGGATGGCCACTTCATGGGA134030.37612890588386505No Hit
ATATGGCTCACCTCTATCCCCGTGTACTTTCCCATCATAATTTTTTATTA133600.3749221952255791No Hit
TGTTCTGTCAGGCTTGATCACCTTTATCCAAAAGAATTTTCTCCTGTGTC132930.3730419716417383No Hit
TTTTAGTGTTCGGTGACCAGTGACTTGGATTTTCCAACACAAGTCATCCC132480.37177913490632275No Hit
GGCATGATTTTGTGCACGGATGGATAAAAGTACCTGTGACTCATGAAACA131980.37037598297808333No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC128350.36018909997906495No Hit
GCACTCTTTCAAAAGTTATTATGGAATACCCTGTATGAAGGGAAGCCAGA127400.35752311131541004No Hit
GACCTGTAGTTGAGGCTGTAATGTCTTACTTCCCTACCAGGTATGCACTC127050.35654090496564245No Hit
ATAATCATACCTGAGCAAAGAGGTAAGACATGACAAAGTCATCAAGAAGA126530.3550816269602734No Hit
CTTGCCAAGTTGCTGTAGAAATTTCAAATGTCTCTCTTCAAACTGTGTAG125920.3533697816078213No Hit
CAGGACTCACACGACTATTCTGGCTTCCCTTCATACAGGGTATTCCATAA125730.35283658387509026No Hit
CTATTGCTGGCACCATCTGACGTGGCAGGCTGGGGGATTTTTATCAAAGA124740.3500583430571762No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA122830.34469830269130153No Hit
GGCAATTCATTTCCAATCAAACCCACAGACTTACCTAATAAAATCAATCT121940.3422006922590353No Hit
GGAAATGCCAATTCTATGAAGCAAATAGATCCAGTTCTTCAGGTGTATCT121530.34105010767787897No Hit
TCTGTAAATCTACTTTGGTCTCAGAATGAAGGTCATTTCTTTCATCCAGC120050.3368967779702902No Hit
TTGGACACAAGCAGTTCTATCATGGTTAAGAGCTGGAAGCATCTTTGATA118870.33358533941964513No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG118230.33178930495149866No Hit
TATGAAACCATTGCTTTTAGAATAATCATGGGCCAGACTGGGAAGAAATC115460.32401584326905214No Hit
CTACCCCAACCAAAGTAACACAACACTTTTAAGGGAAGTGAAAATAGAGG115240.32339845642062676No Hit
TATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAACCCTG114300.3207605307955366No Hit
GCTCATTAAGGGAAGCTTTGTCTTTCGTGTCATTAATTGATGGATATTAT114190.32045183737132393No Hit
TTGCTTCTGTGATTTGAGAGTAAGAGCCTTATGGTCAAATAACGACTTGG112950.31697202058929014No Hit
TTAAAACATCGCCTACAGAAAAGCGTATGAAAGGAGTGTAAGCTTTGCTC112800.3165510750108183No Hit
CTTGTAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCC112510.3157372468924394No Hit
TTTCAGAGAATAAAGAACAGACTACACATCCTGAACTTTTTGCAGGAAAC111580.3131273843059141No Hit
CATCCCCAAGCAGCTTAAACTTCCTTGGGATCTTGCTTCTGGCAAACTTA110130.30905824371401963No Hit
ATATTGAAAGTGGGTTTGTTTTAGGAATTTATGCGTATGATTGGCAATGA109890.30838473078846473No Hit
GGGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGC109220.3065045072046239No Hit
GGAAAAAACACTGTCTCAATATTATCCAGATTGTGTTTCCATTGCGGTGC108580.3047084727364774No Hit
CACAGCTAATGGGTTATCAAAGATGCTTCCAGCTCTTAACCATGATAGAA108330.3040068967723577No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA108330.3040068967723577No Hit
AAAATGCCCAAGATTTAAGACCAAAGGCTTTATCAAGTCACACTTACCCA108260.30381045550240415No Hit
CCCTGCTTCCCTATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGG107810.30254761876698866No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA107120.3006112691060182No Hit
ACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACCCCAGCTTTAGA106700.2994326214862971No Hit
GCCTTCAATTCAATCCATCCTGGTTTCAGATAGTGCTGTGTTGGGGGCAC106600.2991519911006492No Hit
GCATCTGAAGCAGTTTCACGCCAAGTCGTTATTTGACCATAAGGCTCTTA106090.29772077613384496No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC105710.296654380668383No Hit
TGGTGCCTCAGGTTTACCCTCTATTTTCACTTCCCTTAAAAGTGTTGTGT105540.2961773090127816No Hit
CAAAGTCCTCTCAAGTACCCTCTGCAATAATTAGGCACTAAGTCTTACCA104320.2927536183078774No Hit
GGAAGAGGCAGAAACTGTAGCACCATTAGGCATTAGCACTGCCTTGTTTT103730.2910978990325548No Hit
TTGGTTAACCCAAAATCTCCAATTTTAACTCTGTTCTCGTTCTCCACCAA103380.2901156926827872No Hit
ACTTGTGGACTGATATTTGAATATATGTGCGTTTAACTCTAATAGGAAGA102460.28753389313482663No Hit
CTTCTACACAGAGAAATTTGAAGTAAAAGAACCTGGAAGTGGTCCTTCAG102440.28747776705769706No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT102360.28725326274917873No Hit
GCTGATGATGCTGATAATGCCAGTAAACTAGCTGCAATGCTAAATACCTG101940.28607461512945764No Hit
CCAATCATTTCTTGACCAGTGCTTACATTTGGTTTTGTAGAAGCAAATTC101660.2852888500496435No Hit
TAATCAGGATATTAGTTACCTTGCATATCTGAGATGTGTACTGCAGAAGT101120.28377344596714493No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA99780.2800129987994632No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG99370.2788624142183069No Hit
CCAAGCGGAATCCCATCTAAAACGTAATGAGGCATCACTGCCATCAATTC98930.27762764052145616No Hit
TAATTTTTGTTTCCATGCTCTTTAGAATTCAACTAGAGGGCAGCCTTGTG98620.27675768632594766No Hit
TTAGTACTAGAACTGTCCTTGTCTAATATTCTGGAAGACTTTCCATGCTG98100.27529840832057867No Hit
GACAGAGGGTCTTGGCTTGGATACCTATACAGATCCATCGGCTGAGACTG97630.2739794455080336No Hit
TCCACCAATTAAAAGATGGCCCTTAGTGTTCATTTAATTTTGGTTTATTT96710.27139764596007304No Hit
TTGACTGGAGGAAATTGAGAAAGAATTTTGCTACAAATTAAATGTACAAA96500.2708083221502125No Hit
TGTGGTAGTGGCAGAAAAGGAATCCTTAGTGAACACTGAGCTTTGCTTGA94510.2652237774758195No Hit
CACCAAAGTGGGCAGAATTAGCAAACCTTATAAATAATTGTATGGATTAT94360.26480283189734766No Hit
CCTGACTGTTAATGGCATTTATGTGAGATGTGGTTTTCTGCACCGCAATG94130.26415738201035754No Hit
TTGTTCTGGTTTCTGAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTAC94090.26404512985609835No Hit
GCTGAAACCATCTCCCTGCATATTTTGGTTTCCATAACCTAAGTATTTAG94070.2639890037789688No Hit
AACTCACAGATTCTTTCTTATCACTCAAATCGGAGACATTTGGTTGACTG92660.2600321153413335No Hit
TTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAAT92160.2586289634130941No Hit
TTGGTTGTGGTCTTTTCAAGTGAGGTAACCAACAAAAGGGGGTGATATCA91770.2575345049090674No Hit
GCCACAACCCCTTTAAAGAATCCCAATAGGAATCACCCCACCAGGATCTC91610.25708549629203076No Hit
ATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTCAAAACC90050.2527076622759237No Hit
TTTTTTCAGATAAATCAAACCTTCTAGTCTTCAGAACGAATGGTGTTTCT89860.25217446454319264No Hit
ATTCTGAAGGGTCGAGACAAGGAGCAAACACGAGATCTTGTGCCCCCAAC88680.24886302599254756No Hit
GTTTTGGCTCATTCATGCTCTTATGCTGGTAAAAGACGAGGGAGATCCTG88210.2475440631800025No Hit
AGGTAAAATCAAGAGTCCACATATCAAGTAACTGTCTTTTAAATGTTATT88020.24701086544727152No Hit
TGGCAACAGACAAATGGAGTTTTGGTACCACTTTGTGGGAAATCTGCAGT87910.24670217202305886No Hit
AGTAGAAAAAGCCCTTAGAGATCATGCTAGAAATGTACTTTACCAACCTG87840.24650573075310533No Hit
GGCTACAGCTTAAGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTT87570.245748028711856No Hit
CGGACATCTCCTTCCTGTTGTTTCAGATACAGCCAGGCTGATGCCCTGAA87330.24507451578630107No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC87310.2450183897091715No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC85510.2399670427675095No Hit
GCCTGCTGAGCCACATGAAACTTCAGAGCCCACTTACCTGCGTTTCATCA85400.23965834934329683No Hit
GCCTGCAGTGTCTATCTATGTTGACTTCTCTAAGGAGCTTCATGACAGAC84620.2374694323352433No Hit
AACATGGAGTTGACTTTCTAAAAGGTGCTATTTCTTTTTCTTTTCTCTGC84330.2366556042168644No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA84170.2362065955998278No Hit
AATGTATTCGAGCAAAGATCCAAGACTATCATATTTTGACAAGGAAGCGA82980.23286709401061792No Hit
ACGAACTTTCACAGAACAGTAAAACCCAGTTATTAGACGTGTCTTACCAG82900.2326425897020996No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT81550.22885407949585312No Hit
CCTGTGCATCTGACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCT81520.22876989038015877No Hit
TCTCGAACTCGCTTGATTTTGAATACTGATTTTCACCATGAAAACATTCT80560.226075838677939No Hit
ATACACACCTCTGAATAGTTTCTGTGTGCTTTATCCAGAACTTTTAAAAG80550.22604777563937423No Hit
AGAGACTGAGCCATTTTCAAACTCACACCTTTTGCAACATAAGCCTCATA80070.22470074978826438No Hit
GAACGGTATTTAAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAG78870.22133318516048972No Hit
ATGTCTTTATTAGCAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCC78130.21925652030669535No Hit
GCCCTTCTCCGATGCTTTCTGAAAGGCCTCAGAATAATTGTGTGAACAGG77340.21703954026007702No Hit
ACCTGGTGTGAGGGTTTTTGGAACTGGAGATGTTGGTCCACTGTACCTTG77010.21611345998743897No Hit
GCTAACTACAAACAATCTTCCAGAAGTGACTTGTTGCTCACCTTTTTGGA76940.21591701871748548No Hit
AAGAATGGTTTGCCTAAATAAGACTGTCCTCATGGCTCTGTGACTGTGCC76180.2137842277865615No Hit
GAACTATTTATGGACAACAGTCAAACAACAATTCTTTGTACTTTTTTTTT75460.21176368900989673No Hit
ATGAAAACAAAGCAGATATTGTTAAGCTAATAATGAGAGGAATGGAAAGA75220.21109017608434177No Hit
GAGAGCACATCTTTAAACAGCATAAACTACATGAACAATATAAAGAACAT75170.21094986089151782No Hit
GTGAGGCTGCAGTGATTGTGATTCTCATCCTGGTGTGGGAAGGAATCCCG74700.20963089807897275No Hit
AAATGGTGAGTTACATACCATACAGGGCAAGATTGCCTCAAAGGAACAAA74550.20920995250050092No Hit
GGAAAATCCCATATCTGAAGATAAATTTGCTAATTCTGGATAAACGCCAT73480.20620720737406853No Hit
GGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAAG72760.20418666859740373No Hit
TTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTTGG72440.2032886513633305No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG72350.20303608401624737No Hit
TCTCCAGATTATGAACTATTAACAGAAAATGACATGTTACCAAATATGAG71430.2004542844682868No Hit
ATTCACTTTATACAGGAAGAGAAACTGGAGTCTCATTTGCAAAACCTGTC70590.19809698922884453No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA70570.19804086315171496No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT68870.19327014659570085No Hit
TCTAAATACTAGTGAGTTTTCGGTGTAAGAGTAAAACTAACTACTTTCGC66280.18600181960742054No Hit
AGGCACAGGAAAAACATTTGCATGGTTATGTATCAAGTACCTTTGCTGAG66130.1855808740289487No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC65980.18515992845047688No Hit
TCAGAATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGC64650.18142754432135996No Hit
TAGCATAGCATAATTAAAACAATCCATGCATAAAAAGGAAAATGCTGTTT63680.17870542958057545No Hit
TGCCAAACACATACCTTGAATTTAAAATAGGTTTCAATGGGCAGCTTACC61020.1712406613223416No Hit
ATTATTGCTTCTCCTGTGTGTTTCTGACATTTGATGCGTTTCAGAATGTG60090.16863079873581624No Hit
TATTCAAATTGCTTCTTCTTTACCTTTAGGATGGATTTTGCCATTAGTAA59200.16613318830355003No Hit
CAAAGATACCTGTTTCTGTTCTCTCTTACCCTGTCCACAGAACTTTTGCG59060.165740305763643No Hit
GTAGGGATTTCAGGATTTCAATTTCCCTTTCAAAGTCTCTTAGGTGCTCT58750.16487035156813454No Hit
CAGTGATAGCTTTTGTTTTCATTCTGTAGTCTACTTTGTCCCCAGTCCAT58690.1647019733367458No Hit
TACTGAAGAAAGTACCTTATTCTGTAGAGTACATTATGCCTGGTTGACTC56360.15816328535114998No Hit
CACGGTTGCTTCATCTACAGCAAATAATCAGGATATTAGTTACCTTGCAT54820.1538415774121725No Hit
TAATGAAACTTACGATGAGATATTTCCTTCAAATTTTTGGTTTTAGTGGC53130.14909892389472318No Hit
TAATAGGAAGAAAACACCCTTATTCATGGGAATGTATGTGCCAAAAATAT52850.1483131588149091No Hit
CCTCTTTGGAGCAATTCATACTTTCAGTGTATTTTGAAGTGATATATATG51490.1444965855700978No Hit
GCTCACGCTTTGCACACTCAATGAGAATTGACCCATGAGTTGGAGCCACG48940.13734051073607667No Hit
TTAGCCCCTTTTTCCAAGAGAAGAAGCATATGGCTCACCTCTATCCCCGT48110.13501127853519918No Hit
TCTATGTTTGAAGCTCTTTTAACAACATTTTTATCGGATATCTTACCTGT48050.13484290030381046No Hit
GCTTGCAGGTGGATTCTCTTGCTTAGTTACCTTTTTCCATGTTTTGTTTT45980.12903385132089917No Hit
TAGCCAGAGTTTTACATCTAAATACTTAGGTTATGGAAACCAAAATATGC44240.1241508826106259No Hit
ATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTACCT40860.11466557557572726No Hit
TTTTGCCTTTTAGCATTAAGTGAGTTACTTTACTGAAGAAAGTACCTTAT39990.11222409122059063No Hit
CAAAGCAACAAATACTTACACTCTGAACTACATTGACAAAACTTTTCACA36660.10287909937851596No Hit
TATTTTTGAGGATACACTAGATTTTTAACCTGCTTTACAGATGTTATACA36400.10214946037583143No Hit
CGACGGCGGCCAGAACCTTCTCACGCGGTAGCCCGCGCTTCTTGAGATCG35680.10012892159916664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)