FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150896
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN528831.678348000060935No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA92900.2948367702393224No Hit
CAAAGATACCTGTTTCTGTTCTCTCTTACCCNNNN62100.19708679689840602No Hit
TCAAATCTAAAAGCTCAGAGTCTAACCTTNNNNNN61810.1961664237727935No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG61100.1939130964652594No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA60900.19327835637863008No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG54870.17414094276675587No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC54610.17331578065413775No Hit
AGGTAAAATCAAGAGTCCACATATCAAGTAANNNN52490.16658753573586688No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA49380.15671732738878083No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG48220.15303583488633074No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG47990.152305883786707No Hit
CGACTCCTCTGTTGAATGTGTCNNNNNNNNNNNNN45900.14567284988143053No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG44890.14246741244395245No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG44300.14059492918839595No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG44020.13970629306711488No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC41660.13221636004488882No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC39610.12571027415693822No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC38990.1237425798883873No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC37700.11964850632962813No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA37170.11796644510006042No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG36670.11637959488348712No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC35080.11133341119478396No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC35060.11126993718612102No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA34630.10990524599986796No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG34340.10898487287425544No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC34030.10800102573998No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT33400.10600159446709762No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA33050.1048907993154963No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC33020.1047955883025019No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC32460.10301831605993977No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA32460.10301831605993977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)