FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58026912
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA9852431.697906998738792No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA8883521.5309310273136711No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG7059121.2165251874854204No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG6827811.1766626492204169No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC6069271.0459405456557813No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT6065161.0452322536136336No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC6023771.0380993563813976No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA5889251.0149170095420552No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT5578710.9614004619098118No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC5406310.9316901095822573No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT5281810.9102345477215813No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC5249200.904614741518556No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG5204310.8968786758805982No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG5035500.8677870020034842No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA4997710.8612745065599907No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT4752820.8190716748807864No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG4594680.7918188029719728No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG4482590.7725019039441561No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA4438300.7648692386043221No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC4418300.7614225620001974No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC4172610.7190818632568281No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA4075020.7022638047670019No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA3952730.6811891006710817No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC3943280.6795605459756329No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC3888980.6702028189954343No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT3855340.6644055089472968No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC3765100.6488541041094863No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA3759760.647933841456185No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC3614000.6228144623653246No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA3563980.6141943241784088No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT3546080.6111095486177173No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA3522740.6070872770207038No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA3446240.593903739009927No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC3409600.5875894274711706No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT3346470.5767099927702511No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA3254140.5607984102273097No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA3247380.5596334335351155No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA3226020.5559523829219104No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC3205320.5523850726366414No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT3186960.549221023514055No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC3106140.5352930033567873No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA3096940.53370753211889No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG3095030.533378374503196No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA3084620.5315843793307492No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA3005760.5179941334806857No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT2992350.5156831368176201No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA2721580.4690203056126785No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT2589660.4462860267318723No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG2506440.43194440538210954No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT2418550.4167979850452838No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT2366920.40790038939173606No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA2349330.4048690373184084No Hit
TGGGACTCTTAAAGATTTATACCACCCAGATTTTCATTCTTCTTTCTGTT2248990.387577060795515No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC2235930.38532638097302163No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC2209120.3807061109851925No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT1994780.34376807781878865No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT1988240.34264101456923984No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC1977610.3408091059541476No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT1953090.3365834804374908No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT1908000.32881294803349176No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT1883590.3246062792381576No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA1738810.29965578730089926No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC1653730.284993625026953No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT1133460.19533350318555637No Hit
GGAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTT1045550.18018363617212646No Hit
CCTGAAAACAACGTTCTGGTAAGGACAAGGGTTGGGCTGGGGACCTGGAG857060.14770043251655368No Hit
GCCGTGATGAATATCGAACAGAGTTTACCACAGCTTTGCAGCGCGTTGAC782610.13487017885769967No Hit
GGTCACTGATGGAGGAGGTCTTGCCAGCAAAGCAGTAGTTGGGGTTGTAG780930.13458065802295321No Hit
GCTACTGCTGGTCTTACCTGAACACATCTGCAGATTCGTTTTCCCTGTCT780680.13453757456540166No Hit
TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAA772240.13308307703846106No Hit
TCAGTGGACAGGAAACGCACCATATCCCCCTGCCTGGATGGGTGTTTTTG744970.1283835334887371No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN741090.1277148782275369No Hit
GATGTGCAGAGAAGCCAGCAAGTAGTTGAGTGTGACGGGTGCATCGGTTC728010.12546075172843937No Hit
GCGTCTGGAGTTTTTGGGTGTTAATGATTCTGCCTGCCCACAGGTCGGGT717410.12363401312825331No Hit
GCCAATTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAA682440.11760749908594137No Hit
CAGCTGTTACAAACACGAGCCACAACTTTAAAAGAATAAAAAATTAAAAG681390.11742654856422483No Hit
CCCTGGTCATTACAGAATATTGTTGCTATGGTGATCTTTTGAATTTTTTG657480.1133060466839938No Hit
GGAAAGGCGGGAAGAACACCATGATGCGGTCCCACGGCGTGTCTGTGCTG656880.11320264638587005No Hit
GGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGC648460.11175159553553357No Hit
CCTCACAGGCTCATACATAGAAAGAGATGTGACTCCCGCCATCATGGAGG644100.1110002200358344No Hit
GGTACCAGGAGATGATGTAAGGGACAAGCAGCCACACCCCATTCTTGAGG637460.10985592340326503No Hit
CCACTGTCTTTTCCTTTCCTGACCTTTATGGTTGTAATTGCTAAGAAAAA636490.10968875958796498No Hit
TGGTTGGCCGTTATATTGTTCTCCTGTGTCTGTTCTAGCACTCGGGGATA618950.10666602420614767No Hit
GCGTCAGTCTGGTGTGCTAACTCTATGGCCTGCTTATCTGTTCCTCCTCC615360.10604734575570729No Hit
AGCAACAATTTTATTTACTTCTTGACGGTCCAAAGGGAAACTCTAGATGC614610.10591809538305261No Hit
GCTTGAGAAAGTTGGAAACGTTGCCTTAGAAGCCTGTTTTTTCTCCTTTT606490.10451874468177799No Hit
GCCAAGAAACCATATGCTCACCTTGTTACATCACCACACATGGCAATCCC598990.10322624095523125No Hit
GACTACATGGACATGAAGCAGGCTGATACTACACAGTATGTCCCCATGCT592380.10208711433756805No Hit
GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGA589250.10154770944902254No Hit
GCTGCAGTCCTGACATGGGAAACAATCTGGGTGTTCCAGCACAGGCCCAG589100.10152185937449161No Hit
CTCTTTTTCAGCCACAGGAAAAACCCAAGTAGTAATAACAGTGCTGTTGA588210.10136848226560807No Hit
GCATGTTTCCAATTTTAGCGAGTGCCCATTTGACAGAACGGGAAGCCCTC587050.10116857502256885No Hit
CCTGATAATGGCGTGCTTGTGATTCTTTTCCTCTCTCCTAGACCATGCTC583220.10050853645287897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)