FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825184
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG158680.23249190058465824No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT143550.21032400005626223No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG134480.19703498103494352No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG131850.19318160506735055No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT119880.17564361634792555No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA117460.1720979243929541No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT113880.1668526445587401No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA113860.16682334131944282No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC107090.15690419481731188No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA106220.15562950390788No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA106180.15557089742928543No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG103990.15236219272623272No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC103850.15215707005115173No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC99890.14635502867028932No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG97460.1427946850956692No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG97420.14273607861707466No Hit
CTTGGCTTTCTCGATCACGGCGTACAGCGCAAAGCAGAGACGGGCCATGC97350.14263351727953416No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT96380.14121231017361582No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT95120.13936620609788689No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT94930.13908782532456268No Hit
GAGTGAGGCCTTGGCGATTAATGAACCCCCTTACCTTGGAAGGCCGTCTC93520.1370219469541041No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC89690.13141037662867405No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA89320.13086826670167426No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA86590.1268683745375949No Hit
CCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAG85870.1258134579228926No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT85110.1246999348295958No Hit
CCCGGCTACATGTGCCGTTTCATGTGCAGCGCCAGGTGATCGGAGCGCGA83650.12256079836089401No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG82810.12133006231040804No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC81780.11982094548659786No Hit
CGCTCTTGGCCCCGCAAACGCACCGCCACTCACACCTGCAGCTACGCGGG81770.11980629386694923No Hit
TCGCTGTGCCGATCGCCAATGCCCAGCACATATGTGGCCACACAATAGCC80800.1183850867610309No Hit
AGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCGG80450.11787228007332842No Hit
GATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCCTCCAGCTTCGGTCGA78960.1156891887456807No Hit
GCAGCAGGAAGATAGGCACGATGATGAAGAGGATGATCAGCAGCGTCTGG78220.11460496889168115No Hit
TCCCGGCCTGAGTTCCACTTAATGTCTCCAGGGCCTGGACATTGACCAGA77730.11388703952889769No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC77610.11371122009311398No Hit
CCTTATCCTTGAAGTAGGGCACGCTCTTGACCATCCACTCGTAGATCTGC77270.11321306502506012No Hit
ACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCCCC77000.11281747129454679No Hit
GCAGCAGCTAAACCAGTTCCAGCAGCAGCAGCAGCAGCAGCAACAGCAGC76640.11229001298719565No Hit
CAGCGGCTGGAGTTCGACATCAACATCTGCGACCTGCCCCGCATGGCCCG76410.11195302573527688No Hit
GCAGAGCCCACGTTTCATAGCCAGGAAACGGGGCTTCACGGTGAAAATGC75590.1107515929240882No Hit
CAGAAAAGGAAGTGGCCGATCTTGCGGTTGGCCAGGGCCCGGTCCAGCAG74880.10971132792903458No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG74530.1091985212413321No Hit
TGTGGCCAGTACTGAGTTTTGTTATCCTTCCTTTAGGTAAGGAGTGCCAA74160.10865641131433235No Hit
TGGGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGAATGGAGAGA74130.1086124564553864No Hit
GCAGAGCTACCTTTGCCGATGAGGAGGCTGATCTGTGAGTTGATGAGCTT73520.10771870765681922No Hit
TTCATGTAGCAGTGCATTTTCACCGTGAAGCCCCGTTTCCTGGCTATGAA72730.10656122970457646No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA72720.10654657808492783No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA71850.10527188717549593No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT71440.10467117076990158No Hit
GATTCGAGCTGGGAATCAAGGATCCCAGTGCGGGACGCGGCCTCACCCAA70120.10273715697628079No Hit
AACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGCCCA69950.10248807944225388No Hit
CCAGCTCCTCTTCGGGCGACATGATGGCGTCAGCGGCCGGGGCTTCCATC69530.10187271141701087No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG69520.10185805979736225No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG69090.10122804015247062No Hit
GTAGCCCCTCTGCGACAGCTTATAATGGATGTACTTCATCACTATCTCCC68790.10078849156301135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)