FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870621
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG161580.27523493681503197No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC151000.2572129933102478No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG141630.24125216054655887No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG107680.1834218219844204No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC100630.1714128709722532No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA97780.1665581886481856No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG96070.16364537925374506No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA94620.16117545315904397No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG69820.11893119995312251No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA69140.11777288978457305No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC68800.1171937347002983No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA64760.11031200958126917No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA63140.10755250594443075No Hit
TGTGGCCAGTACTGAGTTTTGTTATCCTTCCTTTAGGTAAGGAGTGCCAA62970.1072629284022934No Hit
GATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCCTCCAGCTTCGGTCGA61220.10428198311558522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)