FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7415102
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT169990.22924836367726295No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC169250.2282504003316475No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC168150.22676694130438127No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG166070.22396185514373235No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT165830.22363819135596516No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT155630.20988248037585996No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA143470.19348351512898945No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA143220.19314636535006532No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA142980.1928227015622981No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC136750.1844209290715084No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA133180.17960643022847156No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT131340.17712500785558985No Hit
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACC131000.176666484156253No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG128660.173510762225523No Hit
TGCAGATATACACTTCAGATAACTACACCGAGGAAATGGGCTCAGGGGAC122530.1652438496463029No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC121230.16349067079589735No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT120940.16309957705234532No Hit
GAGTGAGGCCTTGGCGATTAATGAACCCCCTTACCTTGGAAGGCCGTCTC114300.15414487892412No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG113860.1535514953132135No Hit
CCAACAAGCAATAAAAACTGTACAATATTGGTCAGTCTTTTATATCTGAA113170.15262096192338284No Hit
GAGGTAGAAAACAGTACTCCTTTAAAAGCATATCGCTCGAGTGGCAATCC110920.14958661391306555No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG110840.1494787259838098No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG110010.14835938871778162No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG109320.147428855327951No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC109160.1472130794694395No Hit
GTGACCCGTCCGTACTGGTGTTATTACCAGATTCCCCGACCAACTCCAGC109150.14719959347828257No Hit
GGAGTTCTTCCATGTAGAGGACCTAGAAGGTGGCATCAGGAATGTGCTGC108510.14633649004423677No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA108480.14629603207076586No Hit
TTTTCCCTGTCCATTCCATCAAGTCCTGAAAAATCAAAATGGATTTAGAG108460.14626906008845192No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG107970.1456082465217606No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC107130.14447542326457546No Hit
CAGGAAGATTTTATTGAAATTAGCATTTTCTTCACGGAAACAGGGTTCCT106980.14427313339722098No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC106320.14338305798086123No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA106160.14316728212234975No Hit
AGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCGG105210.141886112962438No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT104960.14154896318351387No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA103890.14010596212971851No Hit
GTTCTCAAACTCACACCCTTGCTTGATGATTTCCAGGAGGATGAAGGAGT103760.13993064424467797No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG103300.13931028865145753No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG102730.13854158715551046No Hit
CCATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCT101040.13626245464998324No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC100800.13593879086221605No Hit
GGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGC99610.13433395791453712No Hit
ATTGGGATCAGCATCGACTCCTTCATCCTCCTGGAAATCATCAAGCAAGG99200.13378103227710153No Hit
AACCATGATGTGCTGAAACTGGAACACAACCACCCACAAGTCATTGGGGT98500.1328370128961139No Hit
GCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTCCTC98020.13218968532057954No Hit
CCAGCTCCTCTTCGGGCGACATGATGGCGTCAGCGGCCGGGGCTTCCATC97940.1320817973913238No Hit
AAAGTCAACTATTGCACTTACAGTAAGGCTATCTTATTAGATATGCCAAA96820.13057136638174363No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA93600.12622887722920062No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT93460.1260400733530031No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT92540.12479936216656223No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC92480.12471844621962044No Hit
GCTTTGCCACTTGTACCCGAGTTTTTGATTCTCAACATGTCCGAGACTGC91980.12404414666177216No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA90920.12261463159913377No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA90180.1216166682535183No Hit
CACTTCTCACTTCCGCTTCCTTCCAGTAAGGAGTCGGGGTCTTCCCCAGT88680.1195937695799734No Hit
CTAATAGCCCCATTAAGACCAATAACCCCATGGCCAGGGGTTTCCATCAG88200.11894644200443905No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC87950.11860929222551492No Hit
GCTAATAGCAACACTTCCTATGTTACCTTTCCTTACACAGAATGAAACGG87290.11771921680915516No Hit
TTGACCTTTTCCCCATTTAAGATCTCGACCAAGTCTCCTGTTTTCTGGGA87200.11759784288874245No Hit
ATCTGTGTTAGCTGGAGTGAAAACTTGAAGACTCAGACTCAGTGGAAACA86710.11693702932205113No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG86350.11645153364040037No Hit
CCCCAGTCTCAGAGCTGATCACCAAGGCTGTGGCTGCTTCTAAGGAGCGC85630.11548054227709882No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC85230.11494110263082019No Hit
GACAATATCCGTAATATCACAGCGTGTATTTCTGTCTTTCCAAGGATGTG84720.11425331708181492No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG84240.11360598950628056No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA84210.11356553153280967No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG83440.11252711021372329No Hit
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT83350.1124057362933106No Hit
GTAATAACAAAATAACACGGCATCCCAGTTAATGCTGCGTGCACGGCGGG81710.11019403374356819No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG81160.10945230422993507No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC80240.1082115930434942No Hit
AATACCCTGGCAATATTAATGTGTCTTTTCCCGATATTCCTCAGGTACTC80080.10799581718498276No Hit
AACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGCCCA79630.10738894758291928No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA79280.10691693789242548No Hit
GGTTTCTCCGGCGTCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAG78910.10641795621961776No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT77690.1047726652984679No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG76870.1036668140235967No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG76690.10342406618277132No Hit
GCTTGTTTTCCTGCTCAGAACAAAGTGACTTCCCTGAACACATCTTCATT76450.10310040239500415No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA75820.10225078495211529No Hit
GGGCTTTGTTGCGGTTTTCACACGCCAGCTTCCTACTTCTTTTTGGCAGC75560.10190014918203416No Hit
ACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCCCC74440.10038971817245401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)