FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379751
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG297720.5534085127731748No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA256030.4759142198216981No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG196770.3657604227407551No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC193960.3605371326665491No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC151350.28133272339184473No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA147820.27477108141250406No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC141200.2624656791736272No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA132130.2456061628131116No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG131340.24413769336164445No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA123360.2293042930797355No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC122280.2272967652220335No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA118380.22004735906922088No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA116860.21722194949171442No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC113780.21149677745308285No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC94720.17606762840882412No Hit
ACTGTTGTAACAAGATCTTCCATTTCTAAGCCCTAATAACTATTTGTTTT91800.17063986790466695No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG91210.16954316287129276No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT70960.1319020155393809No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA66800.12416931564304741No Hit
CTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTTTCCATACAGAT66260.12316555171419644No Hit
CACATATGGTGTACGTTCCCCATGTCGCTGTTGGGGTAGAAGCTGAGATA64630.12013567170673885No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA63670.11835120249989266No Hit
TGGAATTATTATGGACAAACATGTTATCAGAGACTGCCAGGACATGGCCA60330.11214273671774028No Hit
GGGACCAGCAACAGAAGCCCTTATACCACCACTTCACCACTGCTATCAAC59680.11093450235893819No Hit
CTTATGGAAGATCGACTGACCAATCGCCTTACAGAGTCTCTGAACATTTT59340.11030250284818015No Hit
GTGTTTTCATGGACTCTTCTCTTTTCCATACAGATGTCCAGAAGGCTTCT57420.10673356443448775No Hit
CTAGAAGGTGATCTACTTCTATACATTGAGGCTGTCTTTTTTTTTTTTTT57220.10636180001639482No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACAAACAGTGCAC55960.1040196841824092No Hit
CATTTTCCACTTCTTCCTCAGTATTTAAGTTCTGACAAACTGCATGCATC54070.10050651043143075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)