FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10375943
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA1040351.0026558549907223No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC1034720.997229842145432No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA912250.8791971968234598No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG790480.7618391889778114No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG671240.6469195137251621No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC663840.6397876318325959No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG662580.638573284375213No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC648630.6251287232399021No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC629150.606354526041633No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT592050.5705987397964696No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG569410.54877903627651No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT564470.5440180232293104No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA523920.5049372379936937No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC521150.5022676011230979No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT515170.49650426953964566No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG499850.48173934648638683No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC498320.48026478171670756No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA488340.4706463788399763No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT477800.4604882659821859No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT473870.45670065843653923No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC468180.4512168195218497No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA459160.4425236337555054No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC452100.4357194329228678No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA447330.4311222604056325No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC440560.4245975522417577No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG438310.4224290746392882No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC393530.3792715515110289No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA389310.3752044512966195No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA385640.37166742338503594No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT362560.34942366202281566No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC355360.34248453369491333No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA354780.34192554835738787No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA345860.33332873937337554No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC340880.3285291756132431No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA336550.32435606093826846No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA328840.316925411020473No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA324300.3125499051026013No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA316360.3048975885854423No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA315880.3044349800302488No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA313460.302102661897815No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT312900.3015629519167559No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC310140.2989029527243933No Hit
TGGGACTCTTAAAGATTTATACCACCCAGATTTTCATTCTTCTTTCTGTT304800.29375643254786576No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT302460.2915012158412975No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA281490.27129100458628197No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA274010.2640820212678501No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG259340.24994354729974905No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG258390.24902796786759523No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT253580.24439224463742715No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT247090.2381373914640819No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA240330.2316223209784402No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT238040.22941529266303795No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT236440.2278732641457263No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT236080.2275263077293312No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC231010.2226400048651No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC203740.19635805632316986No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC195700.18860936302367892No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT191750.18480248012156583No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT188950.18210393021627047No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT178570.17210002021021126No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT175110.16876538354152487No Hit
GGTCACTGATGGAGGAGGTCTTGCCAGCAAAGCAGTAGTTGGGGTTGTAG153000.14745647696792474No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA151530.1460397382676447No Hit
GGAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTT144710.13946684171260385No Hit
GCTACTGCTGGTCTTACCTGAACACATCTGCAGATTCGTTTTCCCTGTCT139950.13487930687360175No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC139830.13476365473480337No Hit
TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAA126980.12237923820514435No Hit
GGAAAGGCGGGAAGAACACCATGATGCGGTCCCACGGCGTGTCTGTGCTG126940.12234068749221155No Hit
GCCGTGATGAATATCGAACAGAGTTTACCACAGCTTTGCAGCGCGTTGAC122090.11766641354911066No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT117980.11370532779526642No Hit
GATGTGCAGAGAAGCCAGCAAGTAGTTGAGTGTGACGGGTGCATCGGTTC110230.10623612716453819No Hit
CCTGAAAACAACGTTCTGGTAAGGACAAGGGTTGGGCTGGGGACCTGGAG109890.10590844610460948No Hit
GCCAATTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAA108450.10452062043902902No Hit
GCGTCTGGAGTTTTTGGGTGTTAATGATTCTGCCTGCCCACAGGTCGGGT108360.10443388133493023No Hit
GGTACCAGGAGATGATGTAAGGGACAAGCAGCCACACCCCATTCTTGAGG106140.10229431676716035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)