FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687837
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG104150.38748629474183144No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA87760.3265078946379561No Hit
CGCCTCCAACGCCATCCTGGCCAGCAGCGCCGCTGGTGAATACCTCGCCA78350.2914983311860057No Hit
CGGCAGGGCGTGTTCGTCAACGTGGCGTCCACGGCGGGCATCCGGCCGCG70930.2638924905044465No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG69970.2603208453488809No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC64800.2410860480006786No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA58780.2186888565043193No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC56920.2117687940154109No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA55050.2048115268894654No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA54730.20362097850427685No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC53910.20057019826723124No Hit
GGGTCTTGGCTGGAGACTTGGCTGCAGCCTTGTGGGCCTTCTTGTGAGCG42230.15711518220784967No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC41530.15451085761524974No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC41330.15376676487450688No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC41290.15361794632635833No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA40760.15164610056338984No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC40740.15157169128931555No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG37840.14078234654854443No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA37530.13962900280039303No Hit
GCCATGCCGTCGAGGAAACCTCCATCGCCGCCACCGTGCCAATCTGCTTA37400.13914534251891017No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG37360.13899652397076162No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA37260.1386244776003902No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG33170.12340778105219923No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT32760.12188239093367641No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC32520.12098947964478501No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT30260.11258123167439096No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT30120.11206036675587099No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC29520.10982808853364247No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA28860.10737258248919113No Hit
TGTGGCCAGTACTGAGTTTTGTTATCCTTCCTTTAGGTAAGGAGTGCCAA28120.10461943934844263No Hit
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACC27660.10290802604473412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)