FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839998
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232521.2636970257576368No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA74970.4074460950501033No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA71810.390272163339308No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA54070.2938590150641468No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG52990.2879894434667864No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG49620.2696742061676154No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC46270.2514676646387659No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC43850.23831547642986567No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC43230.2349459075498995No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT41930.2278806824790027No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG41490.22548937553192994No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG41430.22516328822096546No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC40000.21739154064297897No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT38590.20972848883531395No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA38450.20896761844306352No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT37980.20641326784050854No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA36720.19956543431025467No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG36270.19711977947802117No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG35680.19391325425353725No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC34510.1875545516897301No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT34370.18679368129747967No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA34230.18603281090522925No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC34060.18510889685749657No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC33840.1839132433839602No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT33140.18010889142270808No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC32520.17673932254274188No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC31220.1696740974718451No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA29320.15934799929130358No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT29010.15766321485132048No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA28480.15478277693780101No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT27870.1514675559429956No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA27480.14934798842172653No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC27110.147337116670779No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT26420.1435871125946876No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA26090.14179363238438303No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA25950.1410327619921326No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC25650.13940232543731026No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA25640.1393479775521495No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA25600.13913058601150655No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC24450.1328805792180209No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA23930.13005448918966217No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG23770.12918492302709025No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA23520.12782622589807163No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC23300.12663057242453524No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA22620.12293491623360461No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA22600.12282622046328312No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT21730.11809795445429831No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG21290.11570664750722556No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG20910.11364142787111725No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT20060.10902185763245395No Hit
TCAAATCTAAAAGCTCAGAGTCTAACCTTNNNNNN19450.10570663663764851No Hit
CGACTCCTCTGTTGAATGTGTCNNNNNNNNNNNNN18990.10320663392025427No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT18500.10054358754737777No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT18400.1000001086957703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)