FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615348
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129170.3572823418381854No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG89630.24791527675897315No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG65630.1815316257245499No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG61700.17066130286766307No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA57680.15954204131939717No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC48540.13426093421712101No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC42740.11821821855046873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)