FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001186
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG59490.29727371668600516No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT54700.2733379106190029No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG45230.22601597252829073No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA45030.22501656517685012No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC43340.21657157305717709No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT40980.20477856631017807No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA40090.20033120359626738No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG38630.19303552993075104No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA38470.1922360040495986No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA36830.18404086376778572No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG35540.17759468635099385No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA32510.16245366497666883No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT31890.15935550218720299No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG31790.15885579851148268No Hit
CCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAG31790.15885579851148268No Hit
CTTGTAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCC29350.14666302882390744No Hit
GCAGGCGCCGCTAAAGCTAAGAAGCCCGCGGGGGCCACGCCTAAGAAGGC29100.14541376963460667No Hit
GCCGGCTTCTTCGCTTTCTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGT28960.14471418448859827No Hit
GGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGC28920.14451430301831014No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC28750.14366480676958562No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA27930.13956723662867918No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT27740.13861779964481064No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG27140.13561957759048884No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT27120.1355196368553448No Hit
ATTGTTGTCTTGGTTGCCATGGACGATGACCACCAGGGGCAGAGACAGGG26940.13462017023904824No Hit
TATGAAACCATTGCTTTTAGAATAATCATGGGCCAGACTGGGAAGAAATC26840.13412046656332796No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC26740.13362076288760766No Hit
TCAGTGTCTCACTTTCTTTATGTTTCCCTCTTGACCACTGAATTCCACCG26720.13352082215246358No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA26700.13342088141731953No Hit
GAAAAAGGCAGTGAAGAAGACTCCGAAGAAGGCGAAGAAGCCCGCGGCGG26340.13162194818472644No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG26260.13122218524415022No Hit
ACATATACAAAAGGATAAACGTTAATCTGCCCTGTGCCTTTGCGTTAATT25660.12822396318982843No Hit
TCCACAGGGATCTGGCAACGAGAAATATATTGGTGGAGAACGAGAACAGA25320.12652497069237942No Hit
TTTTCGGCGAAAAGTCAGGATGTTAGCAGAGCCAGTCAAGACTTGCCGAC25290.12637505958966333No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC25030.12507583003279055No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT24990.12487594856250245No Hit
CCAAGCGGAATCCCATCTAAAACGTAATGAGGCATCACTGCCATCAATTC24580.12282716349204922No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG24340.1216278746703205No Hit
GAATAAATCTGCGAAATCTGTACCTTATTCGCTTCCTTGTCAAAATATGA22840.11413231953451602No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC22690.11338276402093558No Hit
ACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCCCC22660.1132328529182195No Hit
CCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGATG22400.11193362336134671No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG21950.10968495682060539No Hit
AGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCGG21810.10898537167459696No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG21630.10808590505830043No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC21350.1066867347662836No Hit
AACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGCCCA21040.10513765337155066No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA21010.10498774226883457No Hit
CGTTTCTCTGGCCGCGCTTAAGAAAGCGCTTGCGGCTGCCGGCTACGATG20650.10318880903624152No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG20600.10293895719838135No Hit
GGAAGAGGCAGAAACTGTAGCACCATTAGGCATTAGCACTGCCTTGTTTT20530.10258916462537715No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCGGCAGCC20480.102339312787517No Hit
CCAAAGAAAGGCCATTGCGCTCCTTAGAAGCAGCCACAGCCTTGGTGATC20320.10153978690636452No Hit
GGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGCCTG20160.10074026102521204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)