FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547192
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG192540.34709452998922696No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG162670.29324746646591643No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC114100.2056896534318625No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA95580.17230339241908338No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA94130.16968945729659257No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT93990.1694370773537314No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA93300.1681932047782013No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA90650.16341601300261466No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG86150.15530380055350526No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT85300.15377149375756238No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG84150.15169837279834553No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC81750.14737185949215387No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC81350.14665077394112191No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG81280.14652458396969134No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC80080.1443613273165955No Hit
CGTTTCTCTGGCCGCGCTTAAGAAAGCGCTTGCGGCTGCTGGCTACGATG78010.14062970959000518No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA77940.14050351961857457No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA77520.13974637978999105No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA75950.13691611900219067No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC73880.13318450127560033No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC71600.12907431363471825No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC71280.1284974451938927No Hit
TGGAATTATTATGGACAAACATGTTATCAGAGACTGCCAGGACATGGCCA71240.1284253366387895No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA69390.12509031596526674No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC68680.12381038911218505No Hit
CCATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCT66410.11971822861007876No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA65280.1176811619284135No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT65010.11719442918146696No Hit
CCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAG62580.11281383445894788No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC62340.11238118312832872No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA60540.10913629814868495No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG58950.10626998308333298No Hit
TTGACCTTTTCCCCATTTAAGATCTCGACCAAGTCTCCTGTTTTCTGGGA58930.10623392880578138No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG58840.10607168455679919No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG58080.10470162200983849No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCAGCAGCC57940.10444924206697731No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC56760.10232203969143307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)