FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8127085
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG197250.24270694843231No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA149160.18353444070044794No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA143540.176619292157028No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG131950.16235833635307126No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA126250.15534475153145316No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG123350.15177643644677027No Hit
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACC120970.14884795717037536No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC119180.1466454454456918No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC116800.14371696616929688No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA116800.14371696616929688No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA116750.14365544349542303No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC114190.14050548259308226No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC112420.13832757993794823No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC112240.13810609831200238No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA107130.13181848104209565No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC104550.12864391107020537No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA103450.12729041224498083No Hit
AATACCCTGGCAATATTAATGTGTCTTTTCCCGATATTCCTCAGGTACTC99770.122762343447866No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT98770.12153188997038915No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG95960.11807431569867918No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT93660.1152442727004824No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA92470.11378003306228494No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT92050.11326324260174465No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA90870.11181130749832197No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT90130.1109007719249891No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT89470.11008867262985438No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCAGCAGCC88930.10942422775201685No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA87730.10794768357904463No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG85170.10479772267670388No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA84420.10387488256859624No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT82540.10156163003093976No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT81780.10062648538805734No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC81350.10009739039274229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)