FastQCFastQC Report
Tue 15 Nov 2022
EGAF00007294584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6561996
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC145360.22151796496066137No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT115760.17640973874412602No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG112830.17194463391931358No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA111210.1694758728898951No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA106640.16251152850443676No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC94670.14427012756484459No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG94570.14411773490870766No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT94320.1437367532683653No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC90960.138616360022164No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG88900.13547707130574294No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG88660.1351113289310143No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG82430.12561726645368268No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA77570.11821098336542724No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG77010.11735758449106033No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT76450.11650418561669346No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC76150.11604700764828262No Hit
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT75720.11539171922689377No Hit
CAGGAAGATTTTATTGAAATTAGCATTTTCTTCACGGAAACAGGGTTCCT74560.11362396441570521No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC74000.11277056554133834No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC73540.1120695593231084No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA73090.11138379237049215No Hit
TGCAGATATACACTTCAGATAACTACACCGAGGAAATGGGCTCAGGGGAC72570.11059135055858005No Hit
ATTGGGATCAGCATCGACTCCTTCATCCTCCTGGAAATCATCAAGCAAGG72000.10972271241859947No Hit
GCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTCCTC71450.10888455280984627No Hit
GTGACCCGTCCGTACTGGTGTTATTACCAGATTCCCCGACCAACTCCAGC69490.10589765674956218No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT69460.1058519389527211No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA68630.10458707990678447No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG68280.10405370561030515No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG67600.10301743554857394No Hit
AACCATGATGTGCTGAAACTGGAACACAACCACCCACAAGTCATTGGGGT66710.10166114090895514No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC66640.10155446604965927No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC66130.10077726350336087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)