FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5142873
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG100270.19496884329051098No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT86710.16860225792081585No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG85380.16601615478352275No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG83030.16144672442815522No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA74420.1447051093814683No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG73000.14194400678375685No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT72710.14138011963352No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT72100.14019401217957356No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA68430.13305792307140388No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG68410.133019034302422No Hit
CCGCTTCACATAGGTCTTTTCCCGAGACAGGCATTTGTCGATCTCCTTGG66950.13018015416674686No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC66850.12998571032183762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65810.12796349433478135No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT65730.12780793925885395No Hit
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT65630.12761349541394468No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA65220.12681627564981673No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC64390.12520239173706993No Hit
CCAGCTCCTCTTCGGGCGACATGATGGCGTCAGCGGCCGGGGCTTCCATC64070.12458017143336031No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA62510.1215468474527759No Hit
GGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGC62470.1214690699148122No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC61260.1191162993914102No Hit
GTCCCCGTCCGCGTCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACC60830.1182801908583004No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG59850.1163746411781897No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG57590.11198021028324051No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA57210.11124132367258534No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT56990.11081354721378497No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA56970.11077465844480314No Hit
GATTCGAGCTGGGAATCAAGGATCCCAGTGCGGGACGCGGCCTCACCCAA56640.11013299375660257No Hit
GAGTGAGGCCTTGGCGATTAATGAACCCCCTTACCTTGGAAGGCCGTCTC56270.10941355153043833No Hit
AGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCGG55710.1083246659989465No Hit
GTGACCCGTCCGTACTGGTGTTATTACCAGATTCCCCGACCAACTCCAGC55510.107935778309128No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC55040.1070218922380545No Hit
GCCGGCTTCTTCGCTTTCTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGT55000.1069441147000908No Hit
ACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCCCC52570.10221912926879587No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT52530.10214135173083216No Hit
GGGCTTTGTTGCGGTTTTCACACGCCAGCTTCCTACTTCTTTTTGGCAGC51800.10072191166299459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)