FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785499
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG113140.40617497977920647No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG92810.33318985215934377No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT76360.27413400615114203No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA63310.22728423165831327No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA63040.22631492597915132No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA61010.21902718328026685No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC59360.213103648574277No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA58790.21105733658493506No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT58760.21094963595391703No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG57360.2059236065064105No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC55390.19885126506956202No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG54850.1969126537112381No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA54600.19601514845275478No Hit
CGTTTCTCTGGCCGCGCTTAAGAAAGCGCTTGCGGCTGCTGGCTACGATG50950.18291157167889846No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT49120.17634183318680066No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC48520.1741878205664407No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA47190.1694130925913095No Hit
CCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAG46740.16779758312603954No Hit
GGTGCAGACCAAAGGTACCGGTGCTTCTGGCTCCTTCAAACTCAACAAGA45720.16413576167142763No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC45490.16331005683362299No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA45300.16262795283717568No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC41900.15042188132180268No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA37710.1353796931896224No Hit
CTGAGAATAGCAAAACCAAATGTATCAGCCTCAACACAAGCCTCCAGGCA37460.13448218793113909No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG37050.13301027930722648No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT36650.13157427089365317No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG36510.13107166794890251No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC36500.13103576773856318No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA36470.13092806710754518No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCAGCAGCC35940.12902535595956055No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG35720.12823555133209527No Hit
TGGAATTATTATGGACAAACATGTTATCAGAGACTGCCAGGACATGGCCA35160.12622513955309264No Hit
GCCGGCTTCTTCGCTTTCTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGT34570.12410702714307204No Hit
TGGATGTTGTGCTGTTGTGTTCAATGTTCTCCTTGCAGAGTCCGAAGTCA34160.12263511851915941No Hit
CTTTGGCTGTCAACTTTCGAATTCGAGTGTGTGAATTAGGAGATGAAATT33310.11958360064031615No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC33120.11890149664386884No Hit
GGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGC33020.11854249454047551No Hit
GCTGCCTTCTTTGCCTTTGTAACCTTCGGCTTCCCCGACTTAGGCTTGGC31960.11473707224450627No Hit
CTTTGTACACTGGTTTTGTCATTTGGAGAGTTGACAGGGATATGTCTTTG31580.11337286425161165No Hit
GCTTTGCCACTTGTACCCGAGTTTTTGATTCTCAACATGTCCGAGACTGC31240.11215225710007434No Hit
GCAGGCGCCGCTAAAGCTAAGAAGCCCGCGGGGGCCACGCCTAAGAAGGC30420.1092084398522491No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC29870.10723392828358581No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACAAACAGTGCAC28790.10335670556693792No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT28450.10213609841540061No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA28160.10109499231555999No Hit
CTTATGGAAGATCGACTGACCAATCGCCTTACAGAGTCTCTGAACATTTT28080.10080779063284533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)