FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272769
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT186260.29693425662574213No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA182270.2905734293738539No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG181780.2897922751499378No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT173740.2769749691085388No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA153710.24504329746560094No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC142400.22701298262378225No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG135680.21630001041007565No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC127930.20394502013385157No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT125200.19959287517203328No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT124900.1991146174839214No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC118170.18838570334727772No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT115530.184177035691893No Hit
TTTTCCCTGTCCATTCCATCAAGTCCTGAAAAATCAAAATGGATTTAGAG114120.18192922455776708No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA113890.18156256033021462No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC112980.18011184534294186No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT109300.17424521770210252No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA104190.16609889508126316No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA101720.16216124011580849No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC100520.1602482093633609No Hit
CCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGG92190.1469685875567871No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC90980.14503961488140246No Hit
GGCCAAGCTTGATACGGCTGTTGTTTTTTTCTACATCGTAGCCAGCAGCC89300.1423613718279758No Hit
GAGGATTGTGGCCTTCTTTGAGTTCGGTGGGGTCATGTGTGTGGAGAGCG89280.14232948798210168No Hit
GGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGC89240.14226572029035345No Hit
GGCACAAGGAAGCGCTCTTGACTCGGCATGGTACCATGACTACCTCCTGG88590.1412294952994443No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA88270.1407193537654583No Hit
AGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCGG88170.14055993453608767No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG87780.13993819954154219No Hit
ATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGT87240.13907733570294076No Hit
GATTGGCTGGGCACGCGTTTAATATAAGTGGAGGCGTCGCGCTGGCGGGC85740.13668604726238126No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG85390.13612807995958404No Hit
GGTTGTTTTCATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCA84950.13542663535035324No Hit
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT83980.1338802688254581No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT82610.13169622538308043No Hit
CCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCG80730.12869914387091252No Hit
GACAATATCCGTAATATCACAGCGTGTATTTCTGTCTTTCCAAGGATGTG80450.12825277002867475No Hit
GTGACCCGTCCGTACTGGTGTTATTACCAGATTCCCCGACCAACTCCAGC79920.12740784811301037No Hit
GCCGGCTTCTTCGCTTTCTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGT79230.126307855430353No Hit
GCAGCAGGAAGATAGGCACGATGATGAAGAGGATGATCAGCAGCGTCTGG78680.12543104966881452No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG78640.12536728197706629No Hit
CCATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCT77870.12413975391091239No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT76490.12193976854559764No Hit
GGGACACTAACACTCTGATCTCCATCCCTCTCCGCCCCCAGGATGACAGC76380.12176440739328995No Hit
GATTCGAGCTGGGAATCAAGGATCCCAGTGCGGGACGCGGCCTCACCCAA73890.11779486858196117No Hit
TTGACCTTTTCCCCATTTAAGATCTCGACCAAGTCTCCTGTTTTCTGGGA72930.11626444398000309No Hit
CCCGGCTACATGTGCCGTTTCATGTGCAGCGCCAGGTGATCGGAGCGCGA72410.11543546398727579No Hit
CCAGCTCCTCTTCGGGCGACATGATGGCGTCAGCGGCCGGGGCTTCCATC72070.11489343860741565No Hit
CTGCTGGTAAAACTCATTTTAGTTGATCTTTCCCTTTCTGGTATTAAACA71890.1146064839945485No Hit
AACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGCCCA70900.11302823362377921No Hit
GAGTGAGGCCTTGGCGATTAATGAACCCCCTTACCTTGGAAGGCCGTCTC69830.11132244786951344No Hit
GTCCTACGCCGACCTCATCACCAAGGCCATCGAGAGCTCGGCGGAGAAGC69770.11122679633189106No Hit
CAGGAAGATTTTATTGAAATTAGCATTTTCTTCACGGAAACAGGGTTCCT68650.10944130096293997No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA68260.1088195659683945No Hit
CGCTCTTGGCCCCGCAAACGCACCGCCACTCACACCTGCAGCTACGCGGG68220.10875579827664625No Hit
CCCCAGTCTCAGAGCTGATCACCAAGGCTGTGGCTGCTTCTAAGGAGCGC67820.10811812135916371No Hit
CCTTATCCTTGAAGTAGGGCACGCTCTTGACCATCCACTCGTAGATCTGC67220.1071616059829399No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG67090.10695436098475809No Hit
TAAATTTGAATAGAATACCTAAAAACAGCATCCCAATTCAAATATTTTCC66780.10646016137370912No Hit
GCTGCCTTCTTTGCCTTTGTAACCTTCGGCTTCCCCGACTTAGGCTTGGC66420.10588625214797483No Hit
TCCCGGCCTGAGTTCCACTTAATGTCTCCAGGGCCTGGACATTGACCAGA66420.10588625214797483No Hit
CTTTGTACACTGGTTTTGTCATTTGGAGAGTTGACAGGGATATGTCTTTG66050.1052964009993035No Hit
ACTGCCTTTTTCGCCCCTGCAGCCTTCTTGGCCTTCTTAGGCGTGGCCCC65960.10515292369286994No Hit
AATACCCTGGCAATATTAATGTGTCTTTTCCCGATATTCCTCAGGTACTC65920.10508915600112166No Hit
CGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTG65910.1050732140781846No Hit
GCAGCAGCTAAACCAGTTCCAGCAGCAGCAGCAGCAGCAGCAACAGCAGC65750.10481814331119159No Hit
GGAGTTCTTCCATGTAGAGGACCTAGAAGGTGGCATCAGGAATGTGCTGC65480.10438771139189089No Hit
GATCGAGCAGATTGACAGTAGCATGTATCCAGGACTGATTTGGGAGAATG65300.10410075677902374No Hit
AACCATGATGTGCTGAAACTGGAACACAACCACCCACAAGTCATTGGGGT64790.1032877187092335No Hit
GACACAGGACATAGTCGCACACAACTTGCCCTCAGAAGCGGCAGGCGAGG64610.10300076409636638No Hit
CGTTTCTCTGGCCGCGCTTAAGAAAGCGCTTGCGGCTGCTGGCTACGATG64380.1026340998688139No Hit
GGGCTTTGTTGCGGTTTTCACACGCCAGCTTCCTACTTCTTTTTGGCAGC63950.1019485971825202No Hit
CCGCTTCACATAGGTCTTTTCCCGAGACAGGCATTTGTCGATCTCCTTGG63890.1018529456448978No Hit
TTTTTAATAGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGT63700.1015500491090936No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC63680.10151816526321948No Hit
GCAGGCGCCGCTAAAGCTAAGAAGCCCGCGGGGGCCACGCCTAAGAAGGC63480.10119932680447823No Hit
GCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTCCTC63210.1007688948851775No Hit
CACTTCTCACTTCCGCTTCCTTCCAGTAAGGAGTCGGGGTCTTCCCCAGT63120.10062541757874394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)