FastQCFastQC Report
Tue 15 Nov 2022
EGAF00007294596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5209405
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG134720.25860918857335913No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA113550.2179711502561233No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC112900.2167234069917774No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG103140.19798806197636773No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG89780.17234213888150374No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC88220.16934755504707352No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG87650.16825338018449323No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA84120.1614771744565838No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC77860.1494604470184215No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA75620.1451605317689832No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC69540.13348933323479362No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC67120.1288438890813826No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA66410.12748096951571244No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC65240.12523503163988978No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT63980.12281632931208074No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA63960.12277793721163932No Hit
GATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCCTCCAGCTTCGGTCGA62980.12089672429001008No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA62480.11993692177897476No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA62120.11924586397102932No Hit
CCACTGCCTTGGTGATAAGCTCAGATACTGGGGGTCCGGATGCTTTGCGT58770.11281518714709263No Hit
CCGCGGCTGAGTGAGGAGGCACACGCCTGTAACCCCAGCCCTTTGGAAGG58130.1115866399329674No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA55890.10728672468352912No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG55200.10596219721830036No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT54960.10550149201300339No Hit
TTGAATTTAAGTAATTTACTACCTTTCGTTCTGTAGCTCTATCATGTTCT54830.10525194336013421No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC54220.10408098429667112No Hit
CTGAGAATAGCAAAACCAAATGTATCAGCCTCAACACAAGCCTCCAGGCA53910.10348590673982921No Hit
GCCGAGAAGACTCCCGTGAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGC53650.10298680943409083No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA53010.10175826221996562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)