FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007294604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966189
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG157130.3163995570849197No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA109350.22018896179746683No Hit
GCCTTTGGGTTGTGTTGGGCTTCTAAGCAGTTGGCCAAAGGAACTTTCTA91810.18487012878486903No Hit
TGGCTGCTTTCGCCTTTTTCGGGCTTTTCGCTTTTTTGGCTCCAGCAGCT89250.17971527060287074No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC88630.17846682838691802No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC88560.178325875233504No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA86160.1734931956878806No Hit
GCTGCATAATTACCCTTCAGCTCTTAGTTCCTCTGCTGGAAACATCATGC83380.1678953418808668No Hit
GGGCTTGGCGGTCTTTGGTTTAGCCGCCTTGGGTTTAACTGCTTTGGCCT83090.1673113931024373No Hit
CCTTCATTTACACAGGGTTCATAGCCATCTCGACACTGCAATGCTAAAAA82670.1664656741819532No Hit
TAAAATATGTTCTGTTTTCTTAACTTCAGGATATGTGTGTAGTTTGTGGC78910.1588944762271432No Hit
GTAACAGGGCAGAACAAGAAAAGGCAGATAGTAAAGAAATTATCCAGCTC78540.15814943813052623No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG77880.1568204512554798No Hit
CGCGTCGGGTTCCTTCAAACTCAACAAGAAGGCGGCCTCTGGGGAAGCCA77520.15609554932363628No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG77090.15522969423837876No Hit
TTTCTCTTAGTAATAGCTGGTTTACCCATGCCAATTTTTGGAGTGACTGA73250.1474974069653813No Hit
AAGATGTTTCATACCAAGGAGGCAAATCTATAAAGTTATCATCTGAGACA72130.14524215651075706No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC68920.1387784476184857No Hit
GAGTCGGCCATGAATTTAATGTGCTTCTGTTTCTTTCTTCAGGCCTGGGC67990.13690578429455666No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG67510.13593924838543198No Hit
GTCCGAGCTCATTACTAAAGCTGTTGCCGCCTCCAAGGAGCGCAGCGGCG66700.13430821903878407No Hit
TTTTCAGAAAGAAGTTGAGAAATTTAAGCGCCTGATTCGAGATCCTGAAA66390.1336839979308077No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC65840.13257650886826902No Hit
CCGCGCCTGCCTTTTTAGCCTTAGGCTTGGCTTCCCCAGAGGCCGCCTTC64650.13018030526023072No Hit
TTGAATTTAAGTAATTTACTACCTTTCGTTCTGTAGCTCTATCATGTTCT64030.12893186304427803No Hit
GAACCAATCATCACTCAGCGTCTCTCTATATAAACCCTCAGCCTCCCTCG61330.12349509855545168No Hit
ACATCTGCCTTGCTTATGTCTGCTGATGATGAAAATGATGACTCTGTCTC61270.12337428156681109No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC60900.12262924347019415No Hit
TGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAA58690.11817915105526591No Hit
CAAGGCCCCTTTCATCCTTAAGGCAAACTTACCCAGCCTCTTTGTTTAAC58670.11813887872571907No Hit
CCCGCCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTT58070.11693070883931321No Hit
CATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGT57410.11560172196426677No Hit
CCACAAAGGCACCAAAAGAAATGAGAGTCACAATCCTGCCCCAGTTTGTT56960.11469559454946239No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA55970.11270211423689272No Hit
CTGCTGGTAAAACTCATTTTAGTTGATCTTTCCCTTTCTGGTATTAAACA55910.11258129724825212No Hit
CAGCTCTTTTGACTGAGACCTGTGGGTGGCTCTGAAAAGAGCCGTTTAAA55180.11111135721979168No Hit
GTGTTTTCACCTTTTTCGCACTCTTGGCCACTTTCTTGGTCCCAGCAGCG54940.11062808926522932No Hit
CTGCATCCACGTCCGCACCGCGTTCATGCCGCTGCCCAGCGGCTCTTCCT53450.10762780071398813No Hit
GCTTCGCAACCTTGGCCTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACA52670.10605717986166054No Hit
CCAACAAGCAATAAAAACTGTACAATATTGGTCAGTCTTTTATATCTGAA52570.1058558182139262No Hit
CTGCTTCCGGCTCGAATTGCTCTCGCTCACGCTTGCCTTCAACATGTCCG52540.10579540971960592No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT51360.10341934227634107No Hit
TTGACCTTTTCCCCATTTAAGATCTCGACCAAGTCTCCTGTTTTCTGGGA51020.10273471267404441No Hit
TGCAGATATACACTTCAGATAACTACACCGAGGAAATGGGCTCAGGGGAC50850.10239239787289608No Hit
GCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTCCTC50690.1020702192365212No Hit
TTTTTACAGACAGTGATGTGTGTTCTGAAATTGTGAACCATGAGTCTAGT50640.10196953841265403No Hit
TGGAATTATTATGGACAAACATGTTATCAGAGACTGCCAGGACATGGCCA50580.10184872142401348No Hit
CAGGAAGATTTTATTGAAATTAGCATTTTCTTCACGGAAACAGGGTTCCT50390.10146613429331827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)