FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439384
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA4516211.5880125954908164No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA4357741.5322905728197207No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG3274751.151484153102613No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG3192071.1224117934481281No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC2920301.0268506518987894No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA2878231.0120577857804516No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC2793680.9823278872706948No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT2763630.9717615543290249No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG2543520.8943653631878946No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT2520150.8861478856222765No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC2496520.8778389855420217No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG2482370.8728634909954449No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC2468340.8679301914556237No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT2404010.8453101515841552No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA2254250.7926507831533903No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT2149940.755972773531241No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG2125360.7473298296475057No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA2050580.7210353079377528No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG2034820.7154936970505409No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC1977050.6951803175483688No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC1952700.6866182474275814No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC1947630.6848355083921649No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA1890490.6647436526754589No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT1821630.6405307512989733No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC1806430.6351860504432867No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC1799230.6326543500379614No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA1730540.6085012249210461No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA1689380.5940283376039368No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT1661700.5842953560456865No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC1640300.5767705798409698No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA1632730.5741087781648154No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA1613820.5674595483502737No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA1594100.5605255022401329No Hit
TAAAGTTTTTAACTATTTTAAAGGCTTGAAGAGTGTCGAATTATGTCCTC1543650.5427860181500415No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA1542890.5425187831072572No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT1533790.539318995094971No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA1524680.5361156908321221No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC1504630.5290656084534039No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC1495470.5258447229377401No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA1489340.5236892613426507No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT1432770.503797831908033No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG1429290.5025741767121257No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT1422340.5001303825708742No Hit
ATGAAAGAGAGATGGTGATTGCATCTAATGTTTTCCTGTTATAGGGCAAA1397100.4912553661499841No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA1381110.48563288149982436No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA1376270.4839310162273557No Hit
GGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTA1276090.44870521808770536No Hit
TGGGACTCTTAAAGATTTATACCACCCAGATTTTCATTCTTCTTTCTGTT1198600.4214577924753926No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT1167590.41055389947967935No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG1151260.40481186231037913No Hit
AGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACATACTT1092500.38415037400247487No Hit
ACACAGGTAGAAGACTGCACTATAGTAATGATTAGAGTAGATTAGTCATT1092030.3839851102260161No Hit
CAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAA1020500.35883336994922255No Hit
ACTCTTCCTTACCATCCCCATTTTTAAAGATGATCTCATTGTTCTGAAAC1012660.35607662950786834No Hit
CACCTTATACTGACTCAGAGGATATTTTTCTAGGAAGTATTCATCACATC1004310.3531405602878037No Hit
CTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTC912950.3210160951446768No Hit
AATTCAATCAGCGGTATAATCAGGAGTTTTTAAAGGTAAACATAATTGTT900220.3165399081780393No Hit
CCTCACGGAGGCATTCTAAAGTCACTATCATTCCATTTGGTAGTAAACAT897750.3156713942889902No Hit
TCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT897550.3156010692777312No Hit
AAGGTAAACATAATTGTTCCTTCTGAAATACTTTACCTCTTTAGCACCCT862420.30324848105008184No Hit
CCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT848640.2984030877743344No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAAGTCA780450.2744257751855666No Hit
TCATCACTGGTACAAAATACTTTTAGTATGGAAAACTCTTCCAGCCAAAC746820.26260062454236No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN566570.19922020814515531No Hit
TAAAATTCCATCATTTAATTGTAAACGTGTTACTCCTCTTTCAGAATGTT524990.1845996383044021No Hit
GGAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTT470270.1653587152239303No Hit
TCAGTGGACAGGAAACGCACCATATCCCCCTGCCTGGATGGGTGTTTTTG388330.13654655811110394No Hit
GGTCACTGATGGAGGAGGTCTTGCCAGCAAAGCAGTAGTTGGGGTTGTAG381180.13403243895859349No Hit
GCCGTGATGAATATCGAACAGAGTTTACCACAGCTTTGCAGCGCGTTGAC378930.13324128258192935No Hit
GCTACTGCTGGTCTTACCTGAACACATCTGCAGATTCGTTTTCCCTGTCT377670.13279823501099744No Hit
CCTGAAAACAACGTTCTGGTAAGGACAAGGGTTGGGCTGGGGACCTGGAG371830.13074474468223363No Hit
TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAA367240.1291307856738388No Hit
GCGTCTGGAGTTTTTGGGTGTTAATGATTCTGCCTGCCCACAGGTCGGGT342550.120449163033911No Hit
GATGTGCAGAGAAGCCAGCAAGTAGTTGAGTGTGACGGGTGCATCGGTTC334840.11773813384987523No Hit
CCTCACAGGCTCATACATAGAAAGAGATGTGACTCCCGCCATCATGGAGG330270.1161312073426063No Hit
GCCAATTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAA329160.11574090353011865No Hit
CAGCTGTTACAAACACGAGCCACAACTTTAAAAGAATAAAAAATTAAAAG321670.11310723185846783No Hit
GGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGC320950.1128540618179353No Hit
GGAAAGGCGGGAAGAACACCATGATGCGGTCCCACGGCGTGTCTGTGCTG318350.11193983667156784No Hit
GGTACCAGGAGATGATGTAAGGGACAAGCAGCCACACCCCATTCTTGAGG312380.10984063508548568No Hit
CCACTGTCTTTTCCTTTCCTGACCTTTATGGTTGTAATTGCTAAGAAAAA305520.1074284871993008No Hit
CCCTGGTCATTACAGAATATTGTTGCTATGGTGATCTTTTGAATTTTTTG305160.10730190217903456No Hit
TGGTTGGCCGTTATATTGTTCTCCTGTGTCTGTTCTAGCACTCGGGGATA294370.10350786782160964No Hit
GCGTCAGTCTGGTGTGCTAACTCTATGGCCTGCTTATCTGTTCCTCCTCC289770.10189039256265184No Hit
GCTTGAGAAAGTTGGAAACGTTGCCTTAGAAGCCTGTTTTTTCTCCTTTT285390.10035027481607899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)