FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007294608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503012
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226511.5070405292838647No Hit
TTAATGAGCTTTTCCATTGCCTCGACTTGCCTATTCAGGTGCTTCAAATA51510.3427118346360508No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA50880.33852025133531866No Hit
GCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGAGCTTTC37210.24756954701625802No Hit
GAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCCCTGGTAGGTTTTCTG34320.2283414902875027No Hit
AGAAAATTGTTAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATA34310.2282749572192371No Hit
CGAGAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAG34200.2275430934683156No Hit
ACATGTCAACCTTTTGAACAGCATGCAAGAATGTTTATGTTTATTTTGTT33150.22055712130042873No Hit
CAATGAAACCCCCAAGAAAGTACCTGAGGTTTCCTAGTTGATGAGCAGGG33090.2201579228908352No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC32880.21876072845725786No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG31370.20871423514915383No Hit
TGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTC30020.19973227093329926No Hit
GGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCT29900.19893387411411217No Hit
TTGTCTTTGTGCATTCTTGGGCTCCTTTACTAATCACTATTAAGAAATCC29550.19660521672481657No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGC28810.19168176967316294No Hit
GCCATGCAGAAACTGACCCTGATTTGTTTTTTTGGAATCACCTAGGTCCT27830.18516152898313518No Hit
ATTGCTCTACTATGAGGTGAATTGAGGTCCCTAAGATCCACAGCTTCTTT26440.17591343249421829No Hit
TCTTTGTAGATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTG26020.1731190436270635No Hit
CAAAGATACCTGTTTCTGTTCTCTCTTACCCNNNN25500.16965932407725287No Hit
TAGTTCACTTTTTCAGGATAACTTTCAACATACAGGTTGCCTTACTGGTT25330.16852826191673786No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC24830.16520160850345839No Hit
GTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCA24740.16460281088906809No Hit
CCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCC24150.1606773598613983No Hit
AGTTCAAACGTTTGTACATTTATGACAAAGCAGGTTGAAACTGGACTTAC23750.15801603713077472No Hit
CGTCTTCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTG23650.15735070644811885No Hit
GACAGGCTGGGAAAGGTTGAGAGCCACTTACCTAGGACGGTGACCTTGGT22960.15275992473779318No Hit
GGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGA22890.15229419325993404No Hit
TTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTATTCCAGA22610.15043126734849754No Hit
GTTTGTCATCTGTTAAATATTTTTCCAAGCACCGAACAGCAAAACCTCGA22220.14783647768613956No Hit
GTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAATATATATTT22150.14737074620828045No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCC21880.14557435336510954No Hit
TTTTCTTCACGGTTGCCTACTGGTTCAATTACTTTTAAAAAGGGTTGAAA21840.14530822109204716No Hit
TATTTATTCCAATAGGTATGGTAAAAACATGCTGAGATCAGCCAAATTCA21270.14151583620090857No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT21100.14038477404039357No Hit
GCTGCAAGTCGGGCCAGAGGTGCCCCTGAACCTGAAGGCCAATGAGACCC20750.13805611665109793No Hit
TCAAATCTAAAAGCTCAGAGTCTAACCTTNNNNNN20610.13712465369537968No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA20190.13433026482822494No Hit
GGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCA20160.13413066562342815No Hit
CGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGT19620.13053787993708635No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC19450.12940681777657131No Hit
AAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAACTCTGTGTTT19010.12647936277288538No Hit
GTCCTACGCCGACCTCATCACCAAGGCCATCGAGAGCTCGGCGGAGAAGC18830.12528176754410478No Hit
GTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCA18670.12421723845185534No Hit
CATTTGCTCCAAACTGACCAAACTGTTCTTATTACTTATAGGTTTCAGGA18550.12341884163266828No Hit
GGTGTAGCTGTGGAAATGCGTCTGGAATAAGATGGCATTGTAAAACAGTC18380.12228777947215326No Hit
ACTGCAGTGAAAAGAGTCTCAAACACAAACTAGAGTCACACACCTTTTGT18360.12215471333562207No Hit
CCCCACATCCCCTGGAATCTCTTGCCCCTTACCTGCCCCACAGGCTCCGC18160.12082405197031028No Hit
CAATAGGGTTCAGCAAATCTTCTAATCCATGAGGTACTGGCCAAAGATTC18010.11982605594632643No Hit
CTTACCAGTCCTGCGTGGGAATAGCTAAATCCTGCTTCTCGGGATACAGA17590.11703166707917169No Hit
GGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAA17270.11490260889467283No Hit
CGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAGCCGGCCGCG17220.11456994355334488No Hit
AGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGGCTCTGGA16840.11204168695925248No Hit
GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTA16760.11150942241312777No Hit
CCTGAATCACTATATTTCCATACTACTCATGAGGTGTTTATTCTTTGTAG16350.1087815666142386No Hit
CCAGGTAGCGGTCCAGACTGATGAAGGCCAGGATGAGGACACTGCTGTAG16200.10778357059025477No Hit
TTTTAATCAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAG16110.10718477297586447No Hit
GATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTTGTGTTC16020.10658597536147416No Hit
TAGACAGAAATATTTTAGAAAGGGACAACAGTTAAGCTTTATGGTTATTT15750.10478958251830324No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTAC15720.10458998331350647No Hit
TCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATATA15440.10272705740206997No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC15380.10232785899247646No Hit
CCTTATCCTTGAAGTAGGGCACGCTCTTGACCATCCACTCGTAGATCTGC15090.10039840001277434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)