FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007294636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243671
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT114240811.152330058237911TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCACCTCGTAT195430.19078121505464202TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT159150.15536422440744144TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTAT146470.1429858495064904TruSeq Adapter, Index 7 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT121770.11887339997545801TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)