FastQCFastQC Report
Wed 8 May 2024
EGAF00007294760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007294760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830784
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT120396710.176561418076775TruSeq Adapter, Index 8 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT205710.17387689607045484TruSeq Adapter, Index 3 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA124150.10493810046739083TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)