FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007326755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007326755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89936392
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7834840.8711534703326769TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG6226820.6923582169051211TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG3967550.44115067457898466TruSeq Adapter, Index 1 (100% over 63bp)
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTATGCACCT2370590.2635851791786355No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAGGCATGTAATAT1745900.1941260885804714No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAATTTTGTCAGCAG1635970.18190300540408602No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTATGCACCTCTATTAGATCATTG1561360.1736071422567185No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAAA1547630.17208050774373962No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTGGGTAGTATTAC1541030.1713466557564373No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACACTTGCCCA1430710.15908020859898406No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAGGCATGTAATATGGTACTGAGCTTCG1412990.15710992720277236No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTG904410.10056107209637673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)