FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007326889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007326889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73092743
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1449240.1982741296218696TruSeq Adapter, Index 21 (97% over 40bp)
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGG1180390.1614920923134599No Hit
GGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTC986620.1349819365788475No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT929670.12719046540639473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)