FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007331310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007331310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77254639
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1724370.22320601355732178TruSeq Adapter, Index 27 (97% over 44bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT1521140.1968995026952362No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCC1111980.14393698739566954No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCAT1011730.13096042038329891No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCA812150.1051263730583221No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAGTCTTCTGCACAGGGCATTCTTTTT801160.10370380476439739No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTTGTTTCTTGATTTGTCTCTCCTTCTC779550.10090656174058363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)