FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007331508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007331508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81951230
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5054940.6168229567756335TruSeq Adapter, Index 10 (100% over 63bp)
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGT1515680.18494902394997612No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACCTTTCAAAGACCTTGCCCTGGACCGT1170540.14283373172068314No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAG1096130.13375394121601347No Hit
GTGGCGGTTAGCGGGGTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCG988690.12064370479857349No Hit
GGCGGTTAGCGGGGTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGC932600.11379939019829231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)