FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074791
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT717801.7615627402730594No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCGTAG711121.7451692614418752No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT684361.6794971815732391No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG641011.5731113571223654No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGATGCGCGGTAGCGGAGCGTTC455691.1183150252368772No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA420801.032691001820707No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT401390.9850566568935684No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGCATCGCAAG358650.8801678417371591No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG272880.6696785184810706No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA254610.6248418630550623No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG247870.608301137408029No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT208770.5123452957464567No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT193370.4745519463452236No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC147460.3618835910847943No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT141320.3468153335962507No Hit
NNNNNNNN117760.28899641723955904No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC101930.25014779899140843No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG100250.24602488814763748No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG91000.22332433737092283No Hit
AAGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGCATCGCAA84820.20815791533847994No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCGTA69530.17063451843296995No Hit
CGGCCCCCCAGATGCGCGGTAGCGGAGCGTTCTGTAAGCCTGCGAAGGGC64990.15949284270039862No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA60670.1488910719592735No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCAAGCGAACCCAGGGAACTGA53980.13247305199211445No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGGTAGCGGAGCGTTCTGTAAG49110.12052151877237384No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTTCAAGCGAACCCAGGGAACTG43910.10776012806546398No Hit
CACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTTG42560.10444707470886236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)