FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4136682
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT1247593.0159195219743746No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA1219912.949005990791654No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA924072.233843452312747No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG651401.5746919874430763No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT550211.3300756500016198No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT431551.0432273981901437No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG397270.9603590510462249No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT387570.9369103063759796No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA266440.6440910855608433No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC235720.5698286694505403No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT226910.5485314075386989No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG215110.5200061305171633No Hit
TGGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTT105610.25530122934274374No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT74270.1795400274906314No Hit
TAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGTA68340.16520486708913085No Hit
TCTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTT58180.14064412009431715No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT57260.13842011544518046No Hit
NNNNNNNN54030.13061192520962453No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG51690.12495521773247256No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAATGGGGCTAGGTATCACTTA50370.12176425454023297No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGCCCAGACTCCTACGGGA44740.10815431304605963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)