FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4136682
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT880532.1285900148959964No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA666521.611243020372366No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG664191.6056104868587915No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA574701.3892776868030947No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT541391.3087542141262005No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG407230.9844363187694872No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT313070.756814277723064No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT306250.7403276345631595No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC268390.6488050084584698No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA213850.5169602111063891No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT140930.34068366869873No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG132760.32093354045585326No Hit
TGGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTT71250.17223949049020448No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT59910.14482621579323718No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT55410.13394793218332954No Hit
TAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGTA51740.12507608755036043No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG50760.1227070391197583No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAATGGGGCTAGGTATCACTTA46230.11175623361911793No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTTCGTGAAGCGAACCCGGCGAA45420.10979814256933457No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTCGCGAGACCTACCAACTCTAAC44600.1078158775559736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)