FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233518
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA920582.8469920377743376No Hit
NNNNNNNN348891.078979612916953No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC240950.7451636267371946No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT189420.5858015944244008No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA151850.4696123540985391No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT110090.340465090962846No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT108970.3370013712618887No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT70040.21660618558486452No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT60860.18821605446451822No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT57500.17782489536164636No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG51740.16001147975672317No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA46200.14287843766448802No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG38460.1189416604453725No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG37930.1173025788011695No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT34240.10589085942926557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)